Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of penehyclidine hydrochloride on IL-6, TNF-α, HIF-1α and oxidative stress in lung tissue of young rats with acute lung injury

Jihong Shu¹, Zhenjiao Fang², Xinjun Xiong³

¹Department of Pediatrics; ²Department of Outpatient Office; ³Department of Respiratory Medicine, Hanchuan People’s Hospital, Hanchuan, PR China.

For correspondence:- Xinjun Xiong Email: pca11n@163.com

Accepted: 27 June 2020 Published: 31 July 2020

Citation: Shu J, Fang Z, Xiong X. Effect of penehyclidine hydrochloride on IL-6, TNF-α, HIF-1α and oxidative stress in lung tissue of young rats with acute lung injury. Trop J Pharm Res 2020; 19(7):1429-1433 doi: 10.4314/tjpr.v19i7.14

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of penehyclidine hydrochloride (PHC) on interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), hypoxia inducible factor-1α (HIF-1α), and oxidative stress levels in lung tissues of acute lung injury (ALI) neonatal rats.

Methods: 40 male Sprague-Dawley (SD) rats were assigned to model, low-dose PHC, high-dose PHC, and control groups (n = 10). Levels of IL-6, TNF-α and HIF-1α were evaluated by enzyme-linked immunosorbent assay (ELISA). Pulmonary lesions were determined histologically using H&E staining.

Results: The lung tissue levels of IL-6, TNF-α and HIF-1α were significantly higher in model rats than in control rats, and significantly lower in PHC-treated rats than in model group, with decrease in levels as PHC dose increased (p < 0.05). The lung tissue activity of MPO and level of MDA in model rats were significantly higher than those in control rats, but significantly lower in the lung tissues of the two PHC groups than in the model group; decrease in levels occurred as PHC dose increased (p < 0.05).

Conclusion: PHC decreases the lung and serum levels of IL-6, TNF-α and HIF-1α in a rat model of ALI, and mitigates pulmonary oxidative stress and lung tissue damage. Thus, penehyclidine hydrochloride may be useful to mitigate ALI-induced damage in patients. However, further studies and clinical trials are required to ascertain this

Keywords: Penehyclidine hydrochloride, Alveolar septum, Acute lung injury, Inflammatory cells, IL-6, TNF-&alpha, HIF-1α, Oxidative stress