Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

A preliminary study of in vitro and in vivo synergistic effects of ciprofloxacin and D-tyrosine against Pseudomonas aeruginosa isolates

Huirong Li, Wei Jiang, Xiaoshuang He, Mengting Chen

Department of Rheumatology, Immunology and Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China;

For correspondence:- Mengting Chen Email: xiaoshuanghe2019@outlook.com Tel:+862782211488

Accepted: 26 July 2020 Published: 31 August 2020

Citation: Li H, Jiang W, He X, Chen M. A preliminary study of in vitro and in vivo synergistic effects of ciprofloxacin and D-tyrosine against Pseudomonas aeruginosa isolates. Trop J Pharm Res 2020; 19(8):1731-1736 doi: 10.4314/tjpr.v19i8.23

© 2020 The authors.
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Abstract

Purpose: To investigate the synergistic antimicrobial effects of ciprofloxacin and D-tyrosine against drug-resistant bacteria.

Method: The antimicrobial effects of ciprofloxacin and D-tyrosine on clinical isolates of multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) no. 3556 were determined in vitro based on time-kill curve, and in vivo in P. aeruginosa-zebrafish infection model. Furthermore, 30 clinical isolates of multidrug-resistant P. aeruginosa were used in vitro to ascertain the synergistic effect of the two agents.

Results: Combined use of ciprofloxacin and D-tyrosine produced synergistic effects against the clinical isolate of P. aeruginosa no. 3556 in vitro and in vivo. Synergism occurred in 96.67 % (95 % CI, range 83.33 - 99.41 %) of the clinical isolates, and ciprofloxacin dose was reduced in 90 % (95 % CI, range 74.38 - 96.54 %) of the clinical isolates in vitro.

Conclusion: These preliminary results suggest that the combination of ciprofloxacin and D-tyrosine is a promising therapeutic strategy against MDR P. aeruginosa infections.

Keywords: Ciprofloxacin, D-tyrosine, Synergistic, P. aeruginosa, Zebrafish infection model, Time-killing curve