Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-inflammatory effect of neo-lignan isoamericanin A via suppression of NF-?B in liposaccharide-stimulated RAW 264.7 cells

Ji Yun Yeo¹, Kwang Woo Hwang², So-Young Park¹

¹Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan 31116; ²Host Defense Modulation Laboratory, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea.

For correspondence:- So-Young Park Email: soypark23@dankook.ac.kr Tel:+82415501434

Accepted: 27 August 2020 Published: 30 September 2020

Citation: Yeo JY, Hwang KW, Park S. Anti-inflammatory effect of neo-lignan isoamericanin A via suppression of NF-?B in liposaccharide-stimulated RAW 264.7 cells. Trop J Pharm Res 2020; 19(9):1857-1862 doi: 10.4314/tjpr.v19i9.9

© 2020 The authors.
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Abstract

Purpose: To investigate the potential anti-inflammatory effects of the seeds of Opuntina humifusa and its active constituents.

Methods: The extract of O. humifusa seeds was tested for the inhibition of nitric oxide (NO) production in liposaccharide (LPS)-stimulated RAW 264.7 cells using Griess reagent. The active constituents were isolated using bioassay-guided isolation methods. The effects of the active constituent on NO, pro-inflammatory cytokines, nuclear factor-kappa B (NF-κB) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (IκB) were evaluated by enzyme-linked immunosorbent assay (ELISA) and western blot analysis.

Results: The seed extract of O. humifusa significantly attenuated LPS-induced NO production in RAW 264.7 cells (p < 0.05). Bioassay-guided fractionation resulted in the isolation of isoamericanin A as an active constituent. Isoamericanin A reduced LPS-induced production of NO, iNOS, and pro-inflammatory cytokines (TNF-α and IL-6) in a concentration-dependent manner (p < 0.05). Furthermore, the effect was accompanied by decreased translocation of NF-κB from the cytosol to the nucleus and the decreased phosphorylation of IκB in the cytosol induced by LPS (p < 0.05).

Conclusion: The seed extract of O. humifusa and its active constituent, isoamericanin A, have anti-inflammatory effects in LPS-stimulated RAW 264.7 cells, suggesting that they have potentials as anti-inflammatory agents.

Keywords: Opuntia humifusa seeds, Isoamericanin A, Nitric oxide, RAW 264.7 cells, NF-kappa B