Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gastroprotective effect of the root extract of Alpinia officinarum Hance (Zingiberoside) against acute indomethacin-induced gastric injuries in rats: Involvement of H+/K+-ATPase and prostaglandin E receptors

Jingwen Gong, Xuguang Zhang, Yinfeng Tan, Hailong Li, Jie Hou , Junqing Zhang

Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, China;

For correspondence:- Jie Hou Email: houjiede20066@163.com Tel:+8689866893826

Accepted: 24 August 2020 Published: 30 September 2020

Citation: Gong J, Zhang X, Tan Y, Li H, Hou J, Zhang J. Gastroprotective effect of the root extract of Alpinia officinarum Hance (Zingiberoside) against acute indomethacin-induced gastric injuries in rats: Involvement of H+/K+-ATPase and prostaglandin E receptors. Trop J Pharm Res 2020; 19(9):1887-1893 doi: 10.4314/tjpr.v19i9.13

© 2020 The authors.
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Abstract

Purpose: To investigate the protective effects of Alpinia officinarum root ethanol extract (AOE) and galangin against acute indomethacin-induced injury on rat gastric mucosa

Methods: Sprague-Dawley rats were daily treated with bismuth potassium citrate (0.08 g/kg), AOE at doses of 0.09, 0.18 and 0.36 g/kg; and galangin (0.2 g/kg) for 15 days. Then, gastric injury on rats was induced by intragastric administration of indomethacin (30 mg/kg). Blood flow and thickness of gastric mucosa were determined using neutral red clearance test and Alcian blue staining. The activity of H+/K+-ATPase was assayed using a biochemical kit. Prostaglandin E receptor expressions were assayed by western blotting.

Results: High doses of ethanol extract of Alpinia officinarum root significantly inhibited H+/K+-ATPase activity by 8.12 % (p < 0.01), increased gastric mucosal blood flow (p < 0.001), enhanced mucus thickness (p < 0.05), and elevated the activities of prostaglandin E receptors 1 and 4 (p < 0.05). Galangin significantly inhibited H+/K+-ATPase activity by 4.82 % (p < 0.05) and increased gastric mucosal blood flow (p < 0.01).

Conclusion: The ethanol extract of Alpinia officinarum root attenuates indomethacin-induced gastric injury by reinforcing gastric mucosal barrier and inhibiting excessive gastric acid secretion. Thus, the extract can be potentially developed for management of gastric injuries.

Keywords: Galangin, Gastric mucosal barrier, Gastric acid, Prostaglandin, Indomethacin