Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of HuChou San on ABCC11 mRNA levels and MRP8 protein expression in skin tissue from bilateral axilla of axillary osmidrosis patients

Bin Chen¹, Huichun Wang¹, Baojin Wu²

¹Department of General Surgery, Huangpu Branch, Shanghai Ninth People's Hospital, Affiliated to Shanghai Jiaotong University, School of Medicine; ²Department of Plastic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.

For correspondence:- Baojin Wu Email: wbshhs@aliyun.com Tel:+862152889999

Accepted: 22 September 2020 Published: 30 October 2020

Citation: Chen B, Wang H, Wu B. Effect of HuChou San on ABCC11 mRNA levels and MRP8 protein expression in skin tissue from bilateral axilla of axillary osmidrosis patients. Trop J Pharm Res 2020; 19(10):2041-2045 doi: 10.4314/tjpr.v19i10.3

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of “HuChou San” on axillary osmidrosis (AO) by measuring ABCC11 mRNA levels and MRP8 protein expression in axillary skin tissues from “HuChou San”-treated AO patients.

Methods: Forty AO patients undergoing treatment at Shanghai Ninth People’s Hospital were enrolled in the study. Tissues from axillary regions were initially treated for 10 min with a solution of copper sulphate and zinc sulphate, followed by treatment with gentle application of “HuChou San” once daily for 3 months. Ten healthy individuals served as untreated control. Axillary skin tissue was surgically collected at 0, 1, 2, and 3 months of treatment. The expressions of ABCC11 mRNA and MRP8 protein in axillary skin samples were determined using qPCR and Western blot, respectively.

Results: The qPCR and Western blot results showed higher levels of ABCC11 mRNA and MRP8 protein in AO patient tissues than in healthy controls. However, “HuChou San” treatment markedly reduced tissue levels of ABCC11 mRNA and MRP8 protein (p < 0.05), when compared to tissues extracted prior to treatment. These effects were time-dependent and increased as treatment duration increased (p < 0.05).

Conclusion: “HuChou San” powder significantly and time-dependently reduced ABCC11 mRNA and MRP8 protein expressions in AO axillary tissue of AO patients. It is known that ABCC11 mRNA and MRP8 protein are two important indices of AO. Therefore, the findings of this study suggest that “HuChou San” has potentials for use as a novel alternative therapy for AO.

Keywords: ABCC11 mRNA, MRP8 protein, Axillary osmidrosis, HuChou San