Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antimicrobial properties of bioactive compounds isolated from epidermal mucus in two Ray species (Dasyatis marmorata and Gymnura altavela)

Ali Uyan¹ , Cemal Turan¹, Elif Ayse Erdogan-Eliuz², Mustafa Kemal Sangun³

¹Molecular Ecology and Fisheries Genetics Laboratory, Faculty of Marine Sciences and Technology, Iskenderun Technical University, 31220 Iskenderun, Hatay; ²Department of Food Technology, Technical Sciences Vocational School, Mersin University, 33343, Mersin; ³Department of Chemistry, Faculty of Arts and Sciences, Hatay Mustafa Kemal University, 31060, Antakya, Hatay, Turkey.

For correspondence:- Ali Uyan Email: ali.uyan@yahoo.com Tel:+905356373437

Accepted: 21 September 2020 Published: 30 October 2020

Citation: Uyan A, Turan C, Erdogan-Eliuz EA, Sangun MK. Antimicrobial properties of bioactive compounds isolated from epidermal mucus in two Ray species (Dasyatis marmorata and Gymnura altavela). Trop J Pharm Res 2020; 19(10):2115-2121 doi: 10.4314/tjpr.v19i10.15

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the occurrence and antimicrobial effects of certain biochemical compounds in the epidermal mucus secretions of fish and to demonstrate their potential for biomedical applications.

Methods: Crude, aqueous, and acidic epidermal mucus samples were collected from live ray specimens. Gas chromatography and gas chromatography-mass spectrometry (GC/MS) analyses were performed to identify the biochemical compounds present in the mucus. The spectrophotometric broth microdilution method was used to determine the antibacterial and antifungal properties of the mucus extracts. The bacterial strains, Bacillus subtilis, Escherichia coli, Enterococcus faecalis, and Klebsiella pneumonia, were used for the tests, as well as the fungal strains, Candida parapsilosis and Candida albicans.

Results: GC/MS analysis revealed the presence of several hydrocarbon-derived compounds in the epidermal mucus of the two ray species. The acidic extract of G. altavela epidermal mucus produced a high MIC value, indicating the highest inhibitory effect of 8.64 µL against E. coli, while the crude extract of G. altavela epidermal mucus (41.13 µL against B. subtilis) was the least effective.

Conclusion: Epidermal mucus extracts, especially when acid-based, displays strong antimicrobial properties against all the tested pathogens. These findings suggest the plants possess some potential for the development of novel antimicrobial components for applications in medicine.

Keywords: Fish, Ray species, Epidermal mucus, Antimicrobial properties, Bioactive compounds