Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Mediator complex subunit 19 regulates the proliferation, migration and invasion of human breast cancer cells

Yanyan Dong , Yunxiao Liu, Liping Zhao, Chongxiao Qu

Department of Pathology, Shanxi Provincial People's Hospital, Taiyuan 030012, Shanxi, China;

For correspondence:- Yanyan Dong Email: dongyanyanren@163.com Tel:+863514960087

Accepted: 17 October 2020 Published: 30 November 2020

Citation: Dong Y, Liu Y, Zhao L, Qu C. Mediator complex subunit 19 regulates the proliferation, migration and invasion of human breast cancer cells. Trop J Pharm Res 2020; 19(11):2273-2278 doi: 10.4314/tjpr.v19i11.4

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the therapeutic implication of mediator complex subunit 19 (Med19) in breast cancer cells.

Methods: The mRNA expression of Med19 was assayed using qRT-PCR. Cell viability was determined with 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay, while 4′,6-diamidino-2-phenylindole (DAPI) and annexin V/propidium iodide (PI) assays were used for determination of apoptosis. Wound healing and Transwell assays were used for the determination of cell migration and invasion. Western blotting analysis was used for assay of protein expression levels.

Results: The results showed that Med19 was significantly (p < 0.05) upregulated in human breast cancer cell lines, relative to normal cells. The up-regulations ranged from 3.7-fold in UACC-2087 cells to 6.4-fold in BT-20 cells. Moreover, Med19 silencing caused significant decrease in the proliferation of BT-20 breast cancer cells (p < 0.05). The inhibition of cell proliferation was due to the induction of apoptosis, as was evident in increased Bax/Bcl-2 ratio. Annexin V/PI staining revealed 6 % apoptosis in si-NC-transfected, and about 13.30 % in si-Med19-transfected BT-20 cells. Wound healing and Transwell assays revealed that the invasion of BT-20 breast cancer cells significantly decreased upon Med19 silencing.

Conclusion: Med19 regulates the proliferation, migration and invasion of human breast cancer cells. Thus, Med19 may be beneficial in the treatment of breast cancer.

Keywords: Breast cancer, Med19, Apoptosis, Migration, Invasion