Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cytotoxic effects of Saccharomyces cerevisiae TC6 and Lactobacillus brevis TBRC 3003 isolated from Thai fermented foods

Vijitra Luang-In¹ , Worachot Saengha¹, Thippiya Karirat¹, Benjaporn Buranrat², Sutisa Nudmamud-Thanoi³, Nyuk Ling Ma⁴, Arjan Narbad⁵

¹Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150; ²Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham 44000; ³Centre of Excellence in Medical Biotechnology, Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; ⁴Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; ⁵Quadram Institute Bioscience, Norwich Research Park, Colney, Norwich NR4 7UA, UK.

For correspondence:- Vijitra Luang-In Email: vijitra.l@msu.ac.th Tel:+6643754085

Accepted: 24 October 2020 Published: 30 November 2020

Citation: Luang-In V, Saengha W, Karirat T, Buranrat B, Nudmamud-Thanoi S, Ma NL, et al. Cytotoxic effects of Saccharomyces cerevisiae TC6 and Lactobacillus brevis TBRC 3003 isolated from Thai fermented foods. Trop J Pharm Res 2020; 19(11):2385-2393 doi: 10.4314/tjpr.v19i11.20

© 2020 The authors.
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Abstract

Purpose: To determine the cytotoxic effect, anti-colony formation effect and antimigratory effect of Saccharomyces cerevisiae TC6 isolated from Thai water kefir, and Lactobacillus brevis TBRC 3003 isolated from picked cabbage.

Methods: Crude microbial extracts were obtained from whole cultures (cells and broths) using ethyl acetate as extracting solvent, and the dried extracts were redissolved in ethanol before use. Cytotoxic, antiproliferative and antimigratory effects of the two microbial extracts on MCF-7, HepG2, and HeLa were tested using 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetra zolium bromide (MTT), clonogenic formation and wound healing assays.

Results: Lb. brevis TBRC 3003 showed the highest cytotoxicity toward HepG2 cells (IC₅₀ of 669.72 µg/mL), while S. cerevisiae TC6 showed the highest cytotoxicity against MCF-7 (IC₅₀ of 691.49 µg/mL) and HeLa (IC₅₀ of 379.16 µg/mL) based on MTT assay. Anti-colony formation test showed that S. cerevisiae TC6 was most the most effective in inhibiting colony formation of HepG2 (IC₅₀ of 311.12 µg/mL) and HeLa (IC₅₀ of 494.64 µg/mL), while Lb. brevis TBRC 3003 was the most potent in inhibiting colony formation of MCF-7 (IC₅₀ of 267.88 µg/mL).

Conclusion: Both microbes can potentially be implemented in functional foods as bio-therapeutics with chemopreventive properties against breast, liver and cervical cancers.

Keywords: Cytotoxicity, Cancer, Lactobacillus brevis, Saccharomyces cerevisiae, Thai fermented foods