Kashif Imran1,
Haroon Khalid Syed2 
,
Sajid Asghar1,
Muhammad Irfan1,
Malik Saad Ullah2,
Ikram Ullah Khan1,
Muhammad Shahid Iqbal3,
Pervaiz A Shah4,
Ikrima Khalid1,
Umar Farooq Gohar5,
Abdullah I Hussain6,
Muhammad Saleem4,
Umar Ashraf1
For correspondence:- Haroon Syed Email: syedharoonkhalid@gcuf.edu.pk Tel:+923166752223
Accepted: 15 October 2020 Published: 30 November 2020
Citation: Imran K, Syed HK, Asghar S, Irfan M, Ullah MS, Khan IU, et al. Validated RP-HPLC method for the simultaneous determination of glucosamine sulphate and curcumin in cream formulation: A novel stability-indicating study. Trop J Pharm Res 2020; 19(11):2415-2424 doi: 10.4314/tjpr.v19i11.24
© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To develop and validate a stability-indicating reverse phase-high performance liquid chromatography (RP-HPLC) method for the simultaneous determination of glucosamine sulphate (GS) and curcumin (Cur) in drug solution and formulation.
Methods: The optimized chromatographic conditions were achieved by passing various compositions of mobile phases over different reverse phase chromatographic columns. Various validation parameters, including linearity, range, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, specificity and system suitability were performed and evaluated. Stability studies under stressed conditions were done to evaluate the effects of acid, alkali, oxidation, heat and degradation by UV light.
Results: The validated method was linear over the concentration range of 0.094 to 1.5 mg/mL for GS and 0.125 to 1.5 mg/mL for Cur, with a correlation coefficient > 0.999. The Intra and inter-day precision were 1.9 % for GS and 0.5 % for Cur, while accuracy was 96 and 102 % for GS and Cur, respectively. Stability studies showed that GS was highly sensitive to acid, alkali and oxidation and less sensitive to heat and UV. Cur was stable against acid, heat and oxidation but sensitive to alkali and UV.
Conclusion: The developed and validated method was precise and accurate for both GS and Cur and can potentially be utilized for their identification and quantification at industrial, research and quality control laboratories.
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