Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-485-5p reduces keratinocyte proliferation and migration by regulating ITGA5 expression in skin wound healing

Keyu Yuan¹, Yi Sun², Yu Ji²

¹Department of Plastic Surgery, Zhuji People's Hospital, Shaoxing City, Zhejiang Province 311800; ²Department of Plastic Surgery, Zhejiang Provincial People's Hospital, Hangzhou City, Zhejiang Province 31000, China.

For correspondence:- Yu Ji Email: yu_ji123@126.com Tel:+8657185333333

Accepted: 19 November 2020 Published: 30 December 2020

Citation: Yuan K, Sun Y, Ji Y. MicroRNA-485-5p reduces keratinocyte proliferation and migration by regulating ITGA5 expression in skin wound healing. Trop J Pharm Res 2020; 19(12):2553-2557 doi: 10.4314/tjpr.v19i12.10

© 2020 The authors.
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Abstract

Purpose: To determine the effect of miR-485-5p on keratinocyte proliferation and migration.

Methods: Human primary keratinocytes (HaCaT cells) were treated with different concentrations of transforming growth factor-β1 (TGF)-β1. miR-485-5p expression levels were determined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) and wound healing assays were performed to investigate the regulatory effects of miR-485-5p on cell viability and migration of HaCaT cells. Downstream target gene expression of miR-485-5p was determined using a luciferase activity assay.

Results: In HaCaT cells, miR-485-5p was time- and dose-dependently downregulated by TGF-β1 treatment (p < 0.05). Forced expression of miR-485-5p decreased cell viability and migration of HaCaT cells (p < 0.05). Knockdown of miR-485-5p enhanced HaCaT cell viability and migration. Integrin subunit alpha-5 (ITGA5) was predicted and verified to be a downstream target of miR-485-5p in HaCaT cells. Overexpression of ITGA5 attenuated the miR-485-5p-induced decrease of HaCaT cell viability and migration (p < 0.05).

Conclusion: MiR-485-5p reduces cell proliferation and migration of keratinocytes through the regulation of ITGA5. This mechanism provides a potential therapeutic strategy for skin wound healing.

Keywords: ITGA5, Keratinocyte, Cell migration, MiR-485-5p, Cell proliferation, Wound healing