Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Bone morphogenetic protein 2/7 promotes repair of bone defect via induction of endochondral ossification

Jianjun Wu , Changkun Zheng, Guiqing Liang

Department of Orthopedics, Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou 350007, PR China;

For correspondence:- Jianjun Wu Email: usehk5@163.com

Accepted: 29 December 2020 Published: 31 January 2021

Citation: Wu J, Zheng C, Liang G. Bone morphogenetic protein 2/7 promotes repair of bone defect via induction of endochondral ossification. Trop J Pharm Res 2021; 20(1):35-40 doi: 10.4314/tjpr.v20i1.6

© 2021 The authors.
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Abstract

Purpose: To study the influence of bone morphogenetic protein 2/7 (BMP2/7) on repair of bone defect, and the mechanism involved.

Methods: Bone marrow stromal cells (BMSCs) were randomly assigned to 2 groups: control and transfection groups. The cells were transfected with rBMP2/7 recombinant adenovirus. Cell growth and alkaline phosphatase (ALP) activity were determined in both groups. Rabbit model of femoral bone defect was prepared using standard methods. Male New Zealand white rabbits were randomly assigned to 3 groups, each of which had 10 rabbits: control, gelatin and BMP2/7 groups. Histopathological and x-ray examinations, and three-point bending flexural test were used to compare the potential of gelatin and BMP2/7 to repair bone defects.

Results: Transfection of BMSCs with rBMP2/7 recombinant adenovirus significantly enhanced their growth (p < 0.05). Alkaline phosphatase (ALP) level was also markedly and time-dependently higher in transfection group than in control group (p < 0.05). Rabbits with grade 4 bone healing or above were more in BMP2/7-treated category than in control and gelatin groups. New bone hyperplasia with typical lamellar bone structure, irregular medullary cavity, as well as transition from osteoblast to osteocyte were observed in BMP2/7 group. Moreover, maximum flexural strength and repair were significantly higher in BMP2/7-transfected group than in control.

Conclusion: These findings indicate that BMP2/7 promotes the repair of bone defect via induction of endochondral ossification in rabbits. Thus, this protein may be useful for the repair of bone defects in humans.

Keywords: Alkaline phosphatase, Bone lesions, BMP2/7, Endochondral ossification