Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Characterization and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates

Azhoma Gumala, Sutriyo , Fadlina Chany Saputri

Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia;

For correspondence:- Sutriyo Email: sutriyo@farmasi.ui.ac.id Tel:+62)8129893150

Accepted: 14 January 2021 Published: 28 February 2021

Citation: Gumala A, S, Saputri FC. Characterization and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates. Trop J Pharm Res 2021; 20(2):223-230 doi: 10.4314/tjpr.v20i2.1

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the characteristics and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates (rsv-PEG-FA-AuNP).

Methods: Gold nanoparticles were produced by citric reduction followed by conjugation of PEG-folic acid and resveratrol. Characterization of rsv-PEG-FA-AuNP conjugates including their particle size, zeta potential, and by Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) was carried out. Biodistribution study of rsv-PEG-FA-AuNP was carried out using female Sprague Dawley rats. Biodistribution data were obtained from high performance liquid chromatography (HPLC) analysis.

Results: The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 ± 10.31 and -36.33 ± 3.12 mV, respectively. TEM images showed rsv-PEG-FA-AuNP conjugates formed spherical shape. Rsv-PEG-FA-AuNP conjugates found in plasma, kidney (1.90 ± 0.20 µg/g), spleen (2.65 ± 1.18 µg/g), liver (1.74 ± 0.03 µg/g), and lung (1.82 ± 0.12 µg/g), after 90 minutes intravenous administration (i.v.) in female Sprague Dawley rats. No free resveratrol was found in plasma, kidney, or spleen after i.v administration in female dawdle Sprague Dawley rats.

Conclusion: Resveratrol-PEG-FA-AuNP conjugates appear to be a potential chemotherapy delivery system for active targeting purposes because of its longer systemic circulation and its accumulation in the kidney.

Keywords: Gold nanoparticle conjugate, PEGylation, Biodistribution, Folic acid, Folate receptor targeting