Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Curcuma longa Linn extract suppresses neuronal apoptosis induction by sevoflurane via activation of the ERK1/2 pathway

Zhou Yu , Yao Yan, Ying Lou

Department of Anesthesiology, The First People’s Hospital of Fuyang District, Hangzhou Fuyang District, Hangzhou 311400, China;

For correspondence:- Zhou Yu Email: ZhouYu7655@gmail.com Tel:+8657163157231

Accepted: 20 January 2021 Published: 28 February 2021

Citation: Yu Z, Yan Y, Lou Y. Curcuma longa Linn extract suppresses neuronal apoptosis induction by sevoflurane via activation of the ERK1/2 pathway. Trop J Pharm Res 2021; 20(2):269-274 doi: 10.4314/tjpr.v20i2.7

© 2021 The authors.
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Abstract

Purpose: To investigate Curcuma longa Linn against neuronal damage induced by exposure to sevoflurane during surgical procedures.

Methods: A sealed box made of transparent glass was used for anaesthetic exposure of neurons. The neurons were exposed to Curcuma longa Linn at doses of 1.5, 3, 6 and 12 µM prior to viability assessment using MTT assay. The effect of Curcuma longa Linn treatment on protein expression was determined using western blotting.

Results: Sevoflurane exposure led to significant and time-dependent reductions in neuronal proliferation, when compared to unexposed cells (p < 0.05). Curcuma longa Linn at doses of 1.5, 3, 6 and 12 µM significantly decreased sevoflurane-mediated neuronal apoptosis. It reduced cleaved caspase-3 and Bax levels in neurons. However, the Curcuma longa Linn-mediated inhibition of sevoflurane-induced neuronal apoptosis was significantly suppressed by VPC23019 (p < 0.05). The p-ERK1/2 level was dose-dependently up-regulated in neurons exposed to sevoflurane on treatment with Curcuma longa Linn. Moreover, VPC23019 reversed the upregulatory effect of Curcuma longa Linn on p-ERK1/2 expression in sevoflurane-exposed neurons (p < 0.05).

Conclusion: Curcuma longa Linn reversed sevoflurane-induced neuronal apoptosis by elevating p-ERK1/2 expression. Therefore, Curcuma longa Linn exerts inhibitory effect on anaesthesia-induced apoptosis in neurons, and may be useful for the treatment of this condition

Keywords: Anesthesia, Neurotoxicity, Sphingomyelin, Apoptosis, Curcuma longa