Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

LncRNA-NEAT1/miR-148a-3p axis regulates cell viability, apoptosis and autophagy through wnt/β-catenin signaling pathway in Breast Cancer

Song An, Yuren Xia, Zilu Gao, Xiaoxuan Sun, Jian Wang

Department of Comprehensive Surgery?Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin, China;

For correspondence:- Jian Wang Email: vby4585@126.com

Accepted: 20 June 2020 Published: 30 May 2021

Citation: An S, Xia Y, Gao Z, Sun X, Wang J. LncRNA-NEAT1/miR-148a-3p axis regulates cell viability, apoptosis and autophagy through wnt/β-catenin signaling pathway in Breast Cancer. Trop J Pharm Res 2021; 20(5):899-910 doi: 10.4314/tjpr.v20i5.3

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: Breast cancer has over the years been one of major acute carcinomas in women. This study investigated the fundamental mechanistic functions of the lncRNA-NEAT1/miR-148a-3p/Wnt/β-catenin axis in moderating cell viability, apoptosis and autophagy in Breast Cancer (BC).

Methods: RT-qPCR measured expression of lncRNA NEAT1 and microRNA-148a-3p in human cell lines for Breast Cancer. Cell transfection upregulated or silenced the genes with CCK-8, western blot and FCM apoptosis assays determining the cellular growth, proliferation and protein expression related to autophagy biomarkers. Furthermore, luciferase assay analyzed the luciferase activity of lncRNA-NEAT1 and microRNA-148a-3p

Results: The outcomes indicated that LncRNA-NEAT1 was upregulated in BC cell lines and promoted cell viability, autophagy and inhibited Apoptosis in BC cells. However, lncRNA-NEAT1 knockdown inhibited cell viability, autophagy and enhanced apoptosis. In addition, lncRNA-NEAT1 directly targeted microRNA-148a-3p. And, it was found that microRNA-148a-3p overturns the cellular viability, autophagy and inhibitory effects on Apoptosis imposed by lncRNA-NEAT1 overexpression. Lastly, overexpressed lncRNA-NEAT1 activated the Wnt/β-catenin regulatory network through sponging microRNA-148a-3p in BC cell lines.

Conclusion The present study showcased that lncRNA-NEAT1 could enhance tumor development in breast cancer via playing the role of molecular sponge to microRNA-148a-3p, and eventually hyper invigorating the Wnt/β-catenin regulatory network

Keywords: lncRNA-NEAT1; miR-148a-3p; Cellular Viability; Apoptosis; Autophagy; Breast Cancer