Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Delicaflavone induces apoptosis and autophagy, and improves the cytotoxicity of multiple myeloma cells to cisplatin by inactivating the AKT/mTOR pathway`

Liangbo Yi¹ , Feifei Yang², Zhonglu Yi²

¹Department of Rehabilitation, Affiliated Hospital of Chifeng University; ²Department of General Practice, Southwest Village Clinic, Xicheng Office, Hongshan District, Chifeng City, Inner Mongolia Autonomous Region 024000, China.

For correspondence:- Liangbo Yi Email: lbyi999@163.com Tel:+8604765973300

Accepted: 27 April 2021 Published: 31 May 2021

Citation: Yi L, Yang F, Yi Z. Delicaflavone induces apoptosis and autophagy, and improves the cytotoxicity of multiple myeloma cells to cisplatin by inactivating the AKT/mTOR pathway`. Trop J Pharm Res 2021; 20(5):967-972 doi: 10.4314/tjpr.v20i5.12

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the effects of delicaflavone in multiple myeloma cells.

Methods: Multiple myeloma cells were incubated with different concentrations of delicaflavone (20, 40, and 80 μM) or coincubated with 40 μM delicaflavone and 20 μM cisplatin. Cell viability and proliferation were investigated using MTT and colony formation assays, respectively. Flow cytometry and western blot analysis were applied to investigate cell apoptosis and autophagy.

Results: Treatment with delicaflavone decreased cell viability and proliferation of multiple myeloma cells but promoted cell apoptosis in a dose-dependent manner. Protein expression of Autophagy-related protein 7 (Atg7) and LC3-II/LC3-I were increased by delicaflavone treatment, while p62 was decreased by the same treatment. Delicaflavone also conferred cisplatin sensitivity in multiple myeloma cells through decreases in cell viability and in cell proliferation. AKT/mTOR phosphorylation was reduced in multiple myeloma cells treated with delicaflavone.

Conclusion: Delicaflavone induces apoptosis and autophagy of multiple myeloma cells, and confers cisplatin sensitivity through inactivation of the AKT/mTOR pathway.

Keywords: Delicaflavone, Apoptosis, Autophagy, AKT/mTOR, Multiple myeloma