Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of resveratrol on endocrine function, oocyte oxidation and apoptosis in mice with polycystic ovary syndrome

Weiyan Huang¹, Lili Duan², Meiyu Wu³, Lei Wang⁴, Zhifang Wang⁵

¹Department of Gynecology, Zhuji People's Hospital, Zhuji 311800, Zhejiang Province; ²Department of Obstetrics, The Eighth People's Hospital of Hengshui, Hengshui 253800, Hebei Province; ³Department of Gynecology, Shangrao Municipal Hospital, Shangrao 334000, Jiangxi Province; ⁴Department of Pharmacy, Deyang People's Hospital, Deyang 618000, Sichuan Province; ⁵Department of Gynaecology, 521 Hospital of Norinco Group, Xi'an 710000, Shaanxi Province, China.

For correspondence:- Zhifang Wang Email: jrsrf1@163.com

Accepted: 27 April 2021 Published: 31 May 2021

Citation: Huang W, Duan L, Wu M, Wang L, Wang Z. Effect of resveratrol on endocrine function, oocyte oxidation and apoptosis in mice with polycystic ovary syndrome. Trop J Pharm Res 2021; 20(5):1009-1015 doi: 10.4314/tjpr.v20i5.18

© 2021 The authors.
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Abstract

Purpose: To investigate the effect of resveratrol on endocrine function, oocyte oxidative stress and oocyte apoptosis in mice with polycystic ovary syndrome (PCOS).

Methods: Healthy female Kunming mice (n = 180) were assigned to control, model and resveratrol groups, respectively. Changes in ovarian tissue in each group, including apoptosis of granulosa cells, were measured. Oocyte abnormal mitochondria, changes in oocyte mitochondrial membrane potential, expression levels of Bcl-2, Bax and caspase-9 in oocytes evaluated. The activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), and levels of reactive oxygen species (ROS) in each group were also assayed.

Results: Compared with the model group, the levels of sex hormones, ovarian cell apoptosis and abnormal mitochondria of mice in the resveratrol group were significantly reduced, while uterine weight increased (p < 0.05). The expression levels of Bax, Bcl-2, caspase-9, GPx and SOD, and ROS levels in the resveratrol group decreased significantly (p < 0.05).

Conclusion: Resveratrol significantly improves endocrine function in PCOS mice, but inhibits oocyte apoptosis and oxidative stress response. Thus, resveratrol may be useful in the treatment of PCOS but further clinical trials are required to confirm it.

Keywords: Resveratrol, Polycystic ovary syndrome, Endocrine function, Oocyte oxidative stress, Apoptosis