Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-21 inhibits the proliferation of childhood cholesteatoma glioma cells by negatively regulating the expressions of PTEN and PDCD4

Xiaozhen Chen¹, Fei Xiao², Shanwu Dong¹, Yongli Chen¹, Jiwu Wang³

¹Department of Pediatrics; ²Department of Abdominal Surgery; ³Department of Cardiothoracic Surgery, Wuhan Fourth Hospital, Wuhan 430000, Hubei Province, China.

For correspondence:- Jiwu Wang Email: qcpp59@163.com

Accepted: 30 May 2021 Published: 30 June 2021

Citation: Chen X, Xiao F, Dong S, Chen Y, Wang J. MiR-21 inhibits the proliferation of childhood cholesteatoma glioma cells by negatively regulating the expressions of PTEN and PDCD4. Trop J Pharm Res 2021; 20(6):1119-1124 doi: 10.4314/tjpr.v20i6.3

© 2021 The authors.
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Abstract

Purpose: To determine the effect of MiR-21 on the proliferation of glioma cells in children with cholesteatoma, and the pathway involved.

Methods: Cholesteatoma tissues from children with cholesteatoma in Otology Department of our hospital were isolated, extracted and cultured in serum-free medium of keratinocytes. The experiment comprised three groups: negative control group, blank control group and miR-21 inhibition group. Cell cycle and cell proliferation were analyzed. mRNA and protein expressions of phosphatase tension homologue (PTEN) and programmed cell death factor-4 (PDCD4) were determined.

Results: The proliferation of CK cells in miR-21 group was significantly lower than that in negative control and blank control groups (p < 0.05). The proportion of CK cells at G0/G1 phase in miR-21 inhibition group was significantly higher than those in negative control and blank control groups (p < 0.05). The protein and mRNA expression levels of PTEN and PDCD4 in CK in miR-21 group were significantly higher than those in negative control and blank control groups (p < 0.05).

Conclusion: Inhibition of miR-21 slows down cell proliferation to some extent, and induces apoptosis of cholesteatoma cells by inducing cell cycle arrest at G0/G1 phase via a mechanism linked to the negative regulation of expressions of PTEN and PDCD4. Thus, MiR-21 can be used as potential target for the drug of treatment of children with cholesteatoma

Keywords: MiR-21, PTEN, PDCD4, Apoptosis, Cholesteatoma, Glioma, Cell proliferation