Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-558 inhibits odontogenic differentiation of human periodontal ligament fibroblasts by negatively regulating Jagged-1/Notch signaling pathway

Shengzhang Song¹, Zhuanjun Yan¹, Wangxi Wu²

¹Department of Orthodontics, Qinghai University Affiliated Hospital, Xining City, Qinghai Province 810001; ²Department of Oral Restoration, Guangdong Stomatological Hospital, Guangzhou City 510280, China.

For correspondence:- Wangxi Wu Email: hongtaoli8@163.com Tel:+86971-6162865

Accepted: 28 May 2021 Published: 30 June 2021

Citation: Song S, Yan Z, Wu W. MiR-558 inhibits odontogenic differentiation of human periodontal ligament fibroblasts by negatively regulating Jagged-1/Notch signaling pathway. Trop J Pharm Res 2021; 20(6):1131-1137 doi: 10.4314/tjpr.v20i6.5

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the potential effect and explore the underlying mechanism of action of miR-558 in the odontogenic differentiation of human periodontal ligament fibroblasts (hPLFs).

Methods: Human periodontal ligament fibroblasts were treated with osteogenic induction medium to induce odontogenic differentiation. The efficiency of odontogenic differentiation was determined using Alizarin red staining and alkaline phosphatase (ALP) activity assays. The expression levels of osteogenic markers, including Osterix and Runx2, were determined by Western blotting, while mRNA levels of Jagged-1, HES1 and HEY1 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: MiR-558 was down-regulated during the odontogenic differentiation of hPLFs. Alizarin red staining and ALP activity data indicate that miR-558 significantly inhibited the odontogenic differentiation of hPLFs. Osterix and Runx2 expression levels were significantly decreased in the miR-558 mimic group but significantly increased in miR-558 inhibitor group compared with those in NC group (p < 0.01). Moreover, miR-558 regulated Notch signaling by targeting Jagged-1, while Jagged-1 knockdown suppressed the odontogenic differentiation of hPLFs.

Conclusion: MiR-558 inhibits the odontogenic differentiation of human periodontal ligament fibroblasts by negatively regulating Jagged-1/Notch signaling pathway, and thus, could serve as a potential target to regulate the odontogenic differentiation of hPLFs.

Keywords: MiR-558, Odontogenic differentiation, Jagged-1, Notch signaling pathway, Human periodontal ligament fibroblasts