Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-612 regulates the proliferation and epithelial-to-mesenchymal transition of human colon cancer cells via G protein-coupled receptor 132 (GPR132)

Sha Shen¹, Qingqing Guo¹, Shuiping Zhan²

¹Department of Pain Management; ²Department of General Surgery, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City 430000, China.

For correspondence:- Shuiping Zhan Email: zsp023@163.com Tel:+862783353610

Accepted: 27 June 2021 Published: 29 July 2021

Citation: Shen S, Guo Q, Zhan S. MicroRNA-612 regulates the proliferation and epithelial-to-mesenchymal transition of human colon cancer cells via G protein-coupled receptor 132 (GPR132). Trop J Pharm Res 2021; 20(7):1345-1350 doi: 10.4314/tjpr.v20i7.4

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and the mechanism involved.

Methods: expressions of miR-612 and GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using cell counting kit-8 (CCK8) and colony formation assays. Dual luciferase assay was used to determine the interaction between miR-612 and GPR132, while cell migration and invasion were measured by Transwell assay.

Results: The expression levels of miR-612 in colon cancer tissues and cell lines were significantly down-regulated (p < 0.05). Overexpression of miR-612 in colon cancer cells led to significant inhibition of their proliferation and colony formation. Transwell assays revealed that miR-612 overexpression markedly stopped the migration, invasion and epithelial-to-mesenchymal transition.

Conclusion: These results indicate that miR-612 exerts anti-cancer effect by suppressing the expression of GPR132 at the translational level. The in vitro tumor suppressive activity of miR-612 against colon cancer reveals its potential for the management of colon cancer.

Keywords: Colon cancer, micro-RNA, G-protein coupled receptor, Epithelial-to-mesenchymal transition