Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Signal processing techniques for the spectrophotometric quantitation of binary mixture of dapagliflozin and saxagliptin: A comparative study

Sherif A Abdel-Gawad^1,2 , Hany H Arab³, Said A Hassan²

¹Pharmaceutical Chemistry Department, College of Pharmacy, Prince Sattam Bin-Abdul Aziz University, Al-Kharj, 11942, Kingdom of Saudi Arabia; ²Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, ET-11562, Egypt; ³Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, PO Box 11099, Taif 21944, Saudi Arabia.

For correspondence:- Sherif Abdel-Gawad Email: sagawad@yahoo.com Tel:+966540586921

Accepted: 25 June 2021 Published: 30 July 2021

Citation: Abdel-Gawad SA, Arab HH, Hassan SA. Signal processing techniques for the spectrophotometric quantitation of binary mixture of dapagliflozin and saxagliptin: A comparative study. Trop J Pharm Res 2021; 20(7):1489-1496 doi: 10.4314/tjpr.v20i7.23

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the advantages and drawbacks of four signal processing methods for spectrophotometric quantitation of mixtures of dapagliflozin and saxagliptin.

Methods: The methods studied were numerical differentiation (ND), Savitzky-Golay filter (SG), discrete Fourier transform (DFT) and continuous wavelet transform (CWT). The resolution powers of the methods were compared via quantitative determination of dapagliflozin (DAP) and saxagliptin (SAX) in laboratory prepared mixtures. Furthermore, a new approach for validating robustness in spectrophotometric methods was developed, and the methods were compared using their robustness.

Results: Continuous wavelet transform (CWT) produced the best results regarding the analysis of the two drugs in different ratios. It also showed a lower limit of quantification (LOQ), when compared to each of the other methods. When the four methods were used for quantitation of pharmaceutical drug formulations, and subjected to validation in line with ICH regulations, they were found to be satisfactorily specific, accurate, precise and robust.

Conclusion: These results show that CWT technique is superior to the other three methods for analysis of drug mixtures with regard to sensitivity and resolving power. Thus, CWT can be used in the routine spectrophotometric analysis of pharmaceuticals in quality control laboratories.

Keywords: Dapagliflozin, Derivative spectrophotometry, Fourier transform, Numerical differentiation, Savitzky-Golay, Saxagliptin, Wavelet transform