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Original Research Article | OPEN ACCESS

Puerarin modulates apoptosis of MC3T3-E1 and ATDC5 cells by attenuating the expression levels of endoplasmic reticulum stress markers

Shuqin Zhang1, Xin Li1 , Yating Bai2, Lixin Fu1, Yanjiang Cui1

For correspondence:-  Xin Li   Email: ai40470770360399@163.com

Accepted: 23 July 2021        Published: 31 August 2021

Citation: Zhang S, Li X, Bai Y, Fu L, Cui Y. Puerarin modulates apoptosis of MC3T3-E1 and ATDC5 cells by attenuating the expression levels of endoplasmic reticulum stress markers. Trop J Pharm Res 2021; 20(8):1565-1571 doi: 10.4314/tjpr.v20i8.3

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the protective effect of puerarin against apoptosis of osteoblasts and chondrocytes.
Methods: Osteoblast-like MC3T3-E1 cells and chondrocyte ATDC5 were used as cellular models. Dexamethasone (Dex) was used to induce cellular stress. Cell viability was determined with cell counting kit-8 (CCK-8) assay. Cell apoptosis and the level of reactive oxygen species (ROS) were also measured. Changes in ERS markers were measured with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting.
Results: Treatment with puerarin reduced cell viability and increased apoptosis, and inhibited the production of ROS. Furthermore, the expression levels of ER stress markers (ATF6, IRE1α, GRP78, XBP1, and eIF2α) were decreased after treatment with puerarin.
Conclusion: Puerarin protected MC3T3-E1 and ATDC5 cells from apoptosis via attenuation of the expression levels of ER stress markers. Thus, puerarin may be a potential drug for treatment of ONFH.

Keywords: Osteoblasts; Chondrocytes; Puerarin; Apoptosis; Endoplasmic reticulum; Osteonecrosis

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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