Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves

Yanqin Zhu^1-3 , Qinhong Yin⁴, Yaling Yang³

¹Research Center for Analysis and Measurement, Kunming University of Science and Technology, China; ²Analysis and Test Center of Yunnan Province, Kunming 650093, China; ³Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; ⁴Yunnan Police College, Kunming 650223, China.

For correspondence:- Yanqin Zhu Email: zyq23788@126.com Tel:+8687165119674

Accepted: 1 November 2021 Published: 30 November 2021

Citation: Zhu Y, Yin Q, Yang Y. A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves. Trop J Pharm Res 2021; 20(11):2371-2379 doi: 10.4314/tjpr.v20i11.20

© 2021 The authors.
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Abstract

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.

Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as the eluate, and at a flow rate of 1.0 and 0.5 mL/min for HPLC-DAD and UPLC-UV methods, respectively. Detector parameter of UPLC-UV was fixed at 203 nm. The assay methods were validated systematically.

Results: The instrumental methods (HPLC-DAD and UPLC-UV) had good linearity, precision, repeatability and recovery. For both methods, quantification limits of UPLC-UV (0.057 - 0.363 μg/mL) were lower than those of UPLC-UV (0.094 - 1.532 μg/mL). The UPLC method with a shorter running time and more sensitive detection was applied in comparing to the HPLC method. After optimization and evaluation, the baseline of 11 compounds was separated effectively within 68 and 34 min, respectively.

Conclusion: The developed HPLC-DAD and UPLC-UV assays were successfully utilized for the simultaneous analysis of eleven major polyphenols and can readily be utilized as quality control tools for Moringa oleifera leaves in China, with UPLC-UV method showing better separation, lower organic solvent usage and shorter analytical period.

Keywords: Moringa oleifera, Polyphenols, HPLC-DAD, UPLC-UV, Validation