Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

LINC00173 mediates albumin paclitaxel resistance in breast cancer cells by regulating β-catenin expression

Chunyu Tian¹, Mengze Xu², Hongxu Zhang¹, Hancheng Liu¹, Xinghua Liu¹, Lihui Ma¹

¹Department of Breast Surgery, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China; ²School of Nursing, Chengde Medical College, Chengde 067000, Hebei Province, China.

For correspondence:- Lihui Ma Email: p072hs@163.com

Accepted: 30 November 2021 Published: 30 December 2021

Citation: Tian C, Xu M, Zhang H, Liu H, Liu X, Ma L. LINC00173 mediates albumin paclitaxel resistance in breast cancer cells by regulating β-catenin expression. Trop J Pharm Res 2021; 20(12):2497-2503 doi: 10.4314/tjpr.v20i12.6

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the effect of LINC00173 on albumin paclitaxel resistance in breast cancer cells, and the underlying mechanism.

Methods: Albumin paclitaxel-resistant SK-BR-3/nab-P cell line was established using human breast cancer cell line SK-BR-3. The cells were transfected to obtain LINC00173 over-expression group, LINC00173 low-expression group and control group. The expression level of LINC00173, and proliferative, invasive and migration ability of cells were measured, and the effect of LINC00173 on albumin paclitaxel resistant cells was determined. The levels of β-catenin, P-glycoprotein (P-gp) and cyclinD1 were assayed.

Results: With increase in albumin paclitaxel concentration, cell viability in the three groups decreased markedly and reached the lowest level at a concentration of 100 μM. Relative to control group, the invasion, migration and population of cell colonies in low LINC00173-expression group were markedly increased, while those in the LINC00173 over-expression group were significantly decreased (p < 0.05). Moreover, compared with the control group, the expressions of β-catenin, P-gp and Cyclin D1 in the low LINC00173-expression group were increased, while those in the LINC00173 over-expression group decreased markedly.

Conclusion: Up-regulation of LINC00173 expression suppressed the proliferation and invasiveness of drug-insensitive mammary tumor cells by suppressing β-catenin, and increasing the sensitivity of drug-resistant breast cancer cells to albumin paclitaxel. This finding may be beneficial for the development of new anti-breast cancer drugs.

Keywords: LINC00173; ?-catenin; breast cancer; albumin paclitaxel; drug resistance