Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Brucea javanica oil emulsion induces autophagy in glioma cells via lncRNA-p21/miR-17-5p/PTEN pathway

Ying Xu, Zeng Wang, Hongyan Zhang, Qiong Wang

Department of Pharmacy, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhe jiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China;

For correspondence:- Qiong Wang Email: wangqiong_0819@163.com Tel:+8657188122425

Accepted: 30 December 2021 Published: 31 January 2022

Citation: Xu Y, Wang Z, Zhang H, Wang Q. Brucea javanica oil emulsion induces autophagy in glioma cells via lncRNA-p21/miR-17-5p/PTEN pathway. Trop J Pharm Res 2022; 21(1):7-12 doi: 10.4314/tjpr.v21i1.2

© 2022 The authors.
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Abstract

Purpose: To investigate the possible effects of Brucea javanica oil emulsion in the progression of glioma, and elucidate the possible regulatory mechanism.

Methods: MTT assays were performed to assess the effects of BJOE on the viability of glioma cells, and flow cytometry (FCM) was conducted to evaluate its effect of the oil on cell apoptosis. Immunoblot assays were carried out to investigate its effects on glioma cell autophagy and LincRNA-p21/miR-17-5p/PTEN axis of glioma cells.

Results: Administration of BJOE (1, 2 and 4 mg/mL) increased the protein levels of LC3 II/ LC3 I and Beclin-1, but decreased the protein levels of p62 (p < 0.05). These changes were reversed by knockdown PTEN. Moreover, the expression of lncRNA-p21 and PTEN mRNA were significantly elevated, while the expression of miR-17-5p significantly decreased in BJOE-treated U251MG cells (p < 0.05).

Conclusion: BJOE may serve as a novel therapeutic drug for the treatment of glioma; The antitumor effect of BJOE is based on its ability to regulate lncRNA-p21/miR-17-5p/PTEN pathway.

Keywords: Glioma, Brucea javanica oil emulsion, Autophagy, Phosphatase and tensin homolog (PTEN)