Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Ophiopogonin B suppresses the growth and epithelial-mesenchymal transition in laryngeal cancer cells by inhibiting FAK/AKT signaling pathway

Rongjie Hu¹, Chun Wu², Xiaoxiao Song², Ling Guo²

¹Department of Otolaryngology-Head and Neck Surgery, The Second Hospital of Jingzhou, Jinzhou, Hubei Province, 434000, China; ²Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong Province 250021, China.

For correspondence:- Ling Guo Email: lguo7825@163.com Tel:+8653168772935

Accepted: 5 January 2022 Published: 31 January 2022

Citation: Hu R, Wu C, Song X, Guo L. Ophiopogonin B suppresses the growth and epithelial-mesenchymal transition in laryngeal cancer cells by inhibiting FAK/AKT signaling pathway. Trop J Pharm Res 2022; 21(1):19-24 doi: 10.4314/tjpr.v21i1.4

© 2022 The authors.
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Abstract

Purpose: To investigate the effect of ophiopogonin B on laryngeal cancer cells, and whether it is related to epithelial mesenchymal transition (EMT).

Methods: Human laryngeal cancer cells (AMC-HN-8) were used as tool cells in vitro. Cell growth was characterized by cell viability and proliferation while cell apoptosis was analyzed using Annexin V/PI staining. Cell invasion and migration were assessed Transwell assay.

Results: Ophiopogonin B inhibited the viability and proliferation of AMC-HN-8 cells at a concentration > 10 μM. Cell apoptosis was enhanced after treatment with ophiopogonin B. The fraction of apoptotic cells for 5, 10 and 20 μM groups were 6.25, 16.16, 28.3 and 39 %, respectively. Transwell assay data showed that ophiopogonin B inhibited the invasion and migration of laryngeal cancer cells. In addition, the expression of N-cad and snail (EMT inducer) was inhibited, while the expression of E-cad was enhanced. These results also indicate that ophiopogonin B inhibited the migration and EMT of laryngeal cancer cells. Besides, ophiopogonin B inhibited the phosphorylation of FAK and AKT.

Conclusion: These results indicate that ophiopogonin B suppresses the growth, migration and EMT in laryngeal cancer cell by inhibiting FAK/AKT signaling pathway. These results provide some ideas for improved treatment of laryngeal cancer.

Keywords: Ophiopogonin B, Laryngeal cancer, Epithelial-mesenchymal transition (EMT), FAK/AKT signaling pathway