Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of cardioprotective potential of hydroalcohol peel extract of Citrullus colocynthis Linn. (Cucurbitaceae)

Ashira Manzoor¹, Imran Ahmad Khan², Saba Kousar¹ , Muhammad Omer Iqbal³, Shaukat Hussain Munawar⁴, Zahid Manzoor³, Perwasha Perwasha⁵

¹Fatima-Tu-Zahra, Department of Life Sciences Muhammad Institute of Medical and Allied Sciences, Multan; ²Department of Pharmacology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; ³Department of Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, Shandong; ⁴Department of Pharmacology and Toxicology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur; ⁵Ali-Ul-Murtaza Department of Rehabilitation Sciences, Muhammad Institute of Medical and Allied Sciences, Multan, Pakistan.

For correspondence:- Saba Kousar Email: sabakousar888@gmail.com Tel:+923316344905

Accepted: 24 December 2021 Published: 31 January 2022

Citation: Manzoor A, Khan IA, Kousar S, Iqbal MO, Munawar SH, Manzoor Z, et al. Evaluation of cardioprotective potential of hydroalcohol peel extract of Citrullus colocynthis Linn. (Cucurbitaceae). Trop J Pharm Res 2022; 21(1):105-110 doi: 10.4314/tjpr.v21i1.17

© 2022 The authors.
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Abstract

Purpose: To investigate the cardioprotective potential of hydroalcoholic peel extract of C. colocynthis against adrenaline-induced myocardial ischemia in rabbits.

Methods: 24 healthy male rabbits were split into 4 groups. Group-I rabbits were administered with subcutaneous injection of adrenaline for two days consecutively. Group-II, Group-III and Group- IV rabbits were given 100, 200 and 300mg/kg of peel extract of C. colocynthis for 14 days orally and the adrenaline was injected on the 14th and 15th day. Histopathology was done to the necrosis level.

Results: Adrenaline-induced group significantly (p< 0.001) elevated the levels of C-reactive protein (CRP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine-kinase (CK-MB) and troponin-I. Groups treated with plant extract showed cardioprotective effects by causing a significant decrease (p < 0.001) of all the above-mentioned biomarkers in comparison with the adrenaline treated group. The cardiac tissues of the adrenaline-treated group showed more necrosis, and mild disintegration of cardiac tissues was observed in groups that had been pre-treated with extract.

Conclusion: Pretreatment of rabbits with peel extract produces a cardioprotective effect against adrenaline-induced myocardial damage. Thus, the extract can potentially be utilized as a therapeutic agent for the treatment of cardiovascular disorders.

Keywords: Adrenaline, Citrullus colocynthis, Cardioprotective, Biomarkers, C-reactive protein