Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Suppressive effect of Hsa-miR-125a-5p on cervical cancer proliferation and invasion via regulation of Rab25-PI3K/AKT pathway

Huanming Lin, Chunjing Chen

Department of Gynecology, Sanming First Hospital, Affiliated Hospital of Fujian Medical University, Sanming, Fujian, China;

For correspondence:- Chunjing Chen Email: jingchen487288@163.com Tel:+8613860556010

Accepted: 1 February 2022 Published: 28 February 2022

Citation: Lin H, Chen C. Suppressive effect of Hsa-miR-125a-5p on cervical cancer proliferation and invasion via regulation of Rab25-PI3K/AKT pathway. Trop J Pharm Res 2022; 21(2):221-227 doi: 10.4314/tjpr.v21i2.2

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the suppressive effect of Hsa-miR-125a-5p on the proliferation and invasion of cervical cancer (CC) cells through the modulation of the Rab25-PI3K/AKT pathway.

Methods: 66 pairs of CC and neighboring cell samples were harvested from patients who underwent surgical resection in the Oncology Department from August 2017 to December 2018. The expression levels of Hsa-miR-125a-5p in CC and neighboring cell samples were assessed by quantitative polymerase chain reaction (qPCR). The overexpression vector was transfected into CC cells (siHa cell line), and the proliferation, invasion, migration, and apoptosis of siHa cells were determined. The regulatory role of Hsa-miR-125a-5p in PI3K/AKT pathway was determined using Western blotting.

Results: CC tissues, relative to the neighboring tissues, exhibited remarkably lower Hsa-miR-125a-5p levels (p < 0.05), and their upregulation significantly inhibited the growth, migration, and intrusion of siHa cells while repressing apoptosis (p < 0.05). Dual-luciferase reporter assay (DLRA) data indicate that luciferase activity decreased when Hsa-miR-125a-5p was bound to Rab25 (p < 0.05). The Hsa-miR-125a-5p mimics group had significantly lower PI3K/AKT levels, relative to the negative control mimics group (p < 0.05).

Conclusion: Hsa-miR-125a-5p represses CC cell proliferation and invasion through the modulation of Rab25-PI3K/AKT pathway, thus suggesting that this pathway is a target for drug development.

Keywords: Hsa-miR-125a-5p, Rab25, PI3K/AKT pathway, Cervical cancer, Cell proliferation