Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Protective effect of Bombyx mori L Cocoon (Abresham) and its formulations against isoproterenol-induced cardiac damage

Tarique Mahmood¹ , Hefazat H Siddiqui¹, Rakesh Dixit², Paramdeep Bagga¹, Md. Sarfaraj Hussain¹

¹Faculty of Pharmacy, Integral University, Dasauli, Kursi Road, Lucknow-226026; ²Department of Pharmacology, King George Medical University, Lucknow-226018, Uttar Pradesh, India.

For correspondence:- Tarique Mahmood Email: ansaritariq79@yahoo.co.in Tel:+91-9918681701

Received: 27 March 2014 Revised: 21 November 2014 Published: 30 January 2015

Citation: Mahmood T, Siddiqui HH, Dixit R, Bagga P, Hussain M. Protective effect of Bombyx mori L Cocoon (Abresham) and its formulations against isoproterenol-induced cardiac damage. Trop J Pharm Res 2015; 14(1):63-72 doi: 10.4314/tjpr.v14i1.10

© 2015 The authors.
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Abstract

Purpose:To investigate the potential activity of Bombyx mori and its formulations against isoproterenol (ISO) induced cardiotoxicity.

Methods:Wistar rats were orally pretreated with the ethanol extract of Bombyx mori cocoons in two doses (250 and 500 mg/kg) for 30 days; rats were similarly pretreated with its polyherbal formulations incorporating Khamira Abresham sada (KAS) and Khamira Abresham Hakim Arshadwala (KAHAW) (800 mg/kg), standard drug metoprolol (10 mg/kg) and normal saline for 30 days. Cardiotoxicity was induced by administration of isoproterenol (ISO, 85 mg/kg, subcutaneous) given twice on days 29 and 30 in all six pre-treated groups (n = 6) except the normal control. Cardiotoxicity was assessed by morphological and biochemical evaluation and further confirmed by histopathological studies.

Results:Pretreatment with Bombyx mori (500 mg/kg), KAHAW and KAS significantly decreased (p < 0.01) the heart weight:body weight (HW:BW) ratio; significantly decreases the elevated activities of the cardiac marker enzymes, namely, asparate transaminase (AST) (p < 0.01), alanine transaminase (ALT) (p < 0.01), lactate dehydrogenase (LDH) (p < 0.01) ,creatinine kinase (CK-MB) (p < 0.01) and thiobarbituric acid reactive substances (TBARS) (p < 0.01) similar to the standard drug metoprolol (p < 0.01) in ISO-injected rats. Pre-treatment of rats with Bombyx mori (500 mg/kg), KAS, KAHAW and metoprolol challenged with ISO also showed absence of troponin. Pretreatment with B. mori (500 mg/kg), KAHAW and KAS significantly increased the activities of Superoxide dismutase (SOD) (p < 0.01), Tissue glutathione (GSH) (p < 0.01) and catalase (p < 0.01) similar to the standard drug metoprolol (p < 0.01).

Conclusion:The findings of this study indicate that Bombyx mori as well as its polyherbal formulations exerts potent cardioprotection against isoproterenol-induced cardiotoxicity. This effect is comparable with that of metoprolol.

Keywords: Bombyx mori, Myocardial necrosis, Oxidative stress, Cardiotoxicity, Khamira Abresham, Metoprolol, Isoproterenol