Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-595 promotes osteogenic differentiation of bone marrow mesenchymal stem cells by targeting HMGA2

Bingjun Gao¹ , Yarong Wu², Lijian Zhou¹, Xin Chen¹

¹Department of Osteology, The People’s Hospital of Danyang; Affiliated Danyang Hospital of Nantong University, Danyang 212300, Jiangsu Province, China; ²Department of Hematology, The People’s Hospital of Danyang; Affiliated Danyang Hospital of Nantong University, Danyang 212300, Jiangsu Province, China.

For correspondence:- Bingjun Gao Email: bingjun_g@163.com Tel:+8651186553701

Accepted: 25 February 2022 Published: 31 March 2022

Citation: Gao B, Wu Y, Zhou L, Chen X. MicroRNA-595 promotes osteogenic differentiation of bone marrow mesenchymal stem cells by targeting HMGA2. Trop J Pharm Res 2022; 21(3):457-463 doi: 10.4314/tjpr.v21i3.1

© 2022 The authors.
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Abstract

Purpose: To investigate the effect of miR-595 on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).

Methods: Human BMSCs were osteogenically differentiated, and protein expression of alkaline phosphatase (ALP), osteocalcin (OCN), and Runt-related transcription factor 2 (RUNX2) were evaluated by western blot. expression of miR-595 was measured by quantitative reverse transcription (qRT-PCR). The effect of miR-595 on viability of BMSCs was determined by MTT assay. Osteogenic differentiation of BMSCs was assessed by ALP and Alizarin red S (ARS) staining. The target gene of miR-595 was predicted by TargetScan analysis and validated by luciferase activity assay.

Results: MiR-595 expression was higher in osteogenically differentiated BMSCs than in undifferentiated BMSCs (p < 0.01). Osteogenic ALP, OCN, and RUNX2 were also upregulated (p < 0.01). MiR-595 expression increased the viability of BMSCs, mineralized bone matrix formation, and ALP activity. High mobility group AT-hook 2 (HMGA2) expression was lower in osteogenically differentiated BMSCs and was found to be a target of miR-595. Overexpression of HMGA2 attenuated the miR-595-induced increase in cell viability, ALP activity, mineralized bone matrix formation, and osteogenic gene expression in BMSCs.

Conclusion: The miR-595/HMGA2 axis is involved in osteogenic differentiation of BMSCs suggesting that it is a promising therapeutic target for osteoporosis.

Keywords: MiR-595, HMGA2, Osteogenic differentiation, BMSCs, Osteoporosis