Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Breviscapine alleviates MPP+-induced damage and apoptosis of SH-SY5Y cells by activating Nrf2 pathway

Dongzhu Li , Xianpei Tan, Qiang Tu, Mingqing Xiang, Yamei Wang, Ming Yu, Tao Tan

Department of Neurology, First People's Hospital of Jingzhou, Jingzhou, Hubei Province, 434000, China;

For correspondence:- Dongzhu Li Email: dzli9053@163.com Tel:+867168110534

Accepted: 11 February 2022 Published: 31 March 2022

Citation: Li D, Tan X, Tu Q, Xiang M, Wang Y, Yu M, et al. Breviscapine alleviates MPP+-induced damage and apoptosis of SH-SY5Y cells by activating Nrf2 pathway. Trop J Pharm Res 2022; 21(3):465-470 doi: 10.4314/tjpr.v21i3.2

© 2022 The authors.
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Abstract

Purpose: To investigate the role and mechanism of action of breviscapine (Brp) in 1-methyl-4-phenylpyridinium ion (MPP⁺)-induced cell injury in human neuroblastoma cell line, SH-SY5Y.

Methods: The injury on SH-SY5Y cells was induced using MPP⁺. Cell viability and apoptotic ability were determined by CCK8 assay and Annexin V/PI staining, respectively. Protein expressions of nuclear factor E2-related factor 2 (Nrf2) and its related downstream proteins - hemeoxygenase 1(HO-1) and NAD(P)H-quinoneoxido reductase 1(NQO1), were determined using Western blotting.

Results: Brp dose-dependently attenuated MPP⁺ induced reduction in the viability of SH SY5Y cells, but alleviated MPP+-induced oxidative stress (OS) and cell injury, as evidenced by the levels of reactive oxygen species (ROS), tyrosine hydroxylase (TH), lactic dehydrogenase (LDH), and dopamine transporter (DAT) (p < 0.05). Brp decreased the amount of apoptotic cells induced by MPP+, as well as the protein levels of Bax and cleaved-caspase 3, and also induced the activation of Nrf2 signaling pathway (p < 0.05).

Conclusion: Brp alleviates MPP⁺-induced cellular damage and cell apoptosis in SH-SY5Y cells by activating Nrf2 pathway. Thus, Brp is a potential therapeutic candidate for the treatment of PD.

Keywords: Breviscapine, Parkinson’s disease, Oxidative stress, Nrf2 pathway, SH-SY5Y cell