Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Polygonum cuspidatum glycoside mitigated LPS-induced human endometrial stromal cell inflammation by regulating NF-κB/Nrf2 signaling pathway-related proteins

Wenjuan Wang , Zhengfang Xiong, Xianghui Zeng

The Reproductive Center, Qinghai Provincial People's Hospital, Xining 810000, Qinghai Province, China;

For correspondence:- Wenjuan Wang Email: Ava1076020@139.com

Accepted: 22 February 2022 Published: 31 March 2022

Citation: Wang W, Xiong Z, Zeng X. Polygonum cuspidatum glycoside mitigated LPS-induced human endometrial stromal cell inflammation by regulating NF-κB/Nrf2 signaling pathway-related proteins. Trop J Pharm Res 2022; 21(3):495-499 doi: 10.4314/tjpr.v21i3.6

© 2022 The authors.
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Abstract

Purpose: To study the influence of polydatin on LPS-provoked human endometrial stromal cell inflammation, and its mechanism of action.

Methods: Fifty ICR female mice were selected and assigned to control and three-dose polydatin groups. Before establishment of the model, mice in low-dose group, middle-dose group and high-dose group were given polydatin at doses of 5, 10 and 20 mg/kg, respectively, by oral gavage for 5 days. Protein expression levels of interleukin 1β (IL-1 β), tumor necrosis factor- α (TNF-α) level, nuclear factor E2-related factor 2 (NRF-2) and nuclear transcription factor kappa-B (NF-2 κB) were determined with Western blot assay.

Results: Model group mice protein levels of NF- κB and nrf-2 were significantly reduced, relative to the corresponding control values (p < 0.05). The NF-κB and NRF-2 proteins in model group were markedly up-regulated, relative to control group, but they were and dose-dependently lower in the 3 polydatin groups than in control (p < 0.05).

Conclusion: Polydatin reduces LPS-induced inflammatory response in mouse endometrial stromal cells, and promotes the repair of endometrium and regeneration of glands via a mechanism related to regulation of NF-κ B/Nrf2 signaling pathway-related proteins.

Keywords: Polydatin, NF- κB, Nrf2 signaling pathway, lipopolysaccharide, Human endometrial stromal cells, Inflammation