Guo Huang1,
Yamei Li2,
Qian Yu2,
Rongjian Feng2,
Guanlan Zhao3 
1Department of Medical Genetics, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China;
2Department of Rehabilitation Medicine, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China;
3Department of Orthopedics, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China.
For correspondence:- Guanlan Zhao
Email: 104071228@qq.com Tel:+8613550065584
Accepted: 14 February 2022
Published: 31 March 2022
Citation:
Huang G, Li Y, Yu Q, Feng R, Zhao G.
Probucol reduces the cerebral edema area and infarction volume in rat cerebral infarction model via PI3K/Akt pathway. Trop J Pharm Res 2022; 21(3):571-577
doi:
10.4314/tjpr.v21i3.17
© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To study the effects of probucol on rats with cerebral infarction through the phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (Akt) pathway.
Methods: Sprague-Dawley (SD) rats were divided into sham group (SO group, n = 7), model group (MO group, n = 7) and probucol group (PR group, n = 7). Infarction volume, messenger ribonucleic acid (mRNA), protein expressions of PI3K/Akt, neurological score, brain water content, degree of brain tissue lesions and neurological function score were determined.
Results: Neurological score was 0, 2.54 ± 0.67 and 1.34 ± 0.21 points, in SO, O and PR groups, respectively. In turning angle test, neurological function score gradually rose at 24 h after cerebral infarction in PR and MO groups, compared with that in the SO group (p < 0.05), but significantly declined at 48 h in PR group compared with that in MO group (p < 0.05). Brain water content was lowest in the SO group but highest in MO group; it was significantly lower in PR group than that in MO group (p < 0.05). The mRNA and protein expressions of PI3K/Akt were highest in SO group and lowest in MO group; the expressions were higher in PR group than those in the MO group (p < 0.05).
Conclusion: Probucol reduces the cerebral edema area and infarction volume by activating PI3K/Akt pathway, thereby exerting a significant therapeutic effect on rat model with cerebral infarction. Thus, this agent has the potential for use in the management of cerebral infarction.
Keywords: Probucol, Signaling pathway, Cerebral infarction, Molecular mechanism
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