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Original Research Article | OPEN ACCESS

Hsc70 interacts with KCNA5 to promote the proliferation of granulosa cells in polycystic ovarian syndrome

Lu Deng1, Jiaoqin Wang2

1Department of Obstetrics, Wuhan, Hubei 430015, China; 2Department of Family Planning, Chunan County Maternal and Child Health Hospital, Hangzhou, Zhejiang 311700, China.

For correspondence:-  Jiaoqin Wang   Email: wangjiaoqing10621@163.com   Tel:+8657165023620

Accepted: 29 March 2022        Published: 28 April 2022

Citation: Deng L, Wang J. Hsc70 interacts with KCNA5 to promote the proliferation of granulosa cells in polycystic ovarian syndrome. Trop J Pharm Res 2022; 21(4):693-699 doi: 10.4314/tjpr.v21i4.2

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the function and interaction of heat-shock cognate protein 70 (Hsc70) and potassium voltage?gated channel subfamily A member 5 (KCNA5) in polycystic ovary syndrome (PCOS).
Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot were used to measure Hsc70 and KCNA5 expression in PCOS. The effect of Hsc70 on granulosa cell apoptosis was determined by flow cytometry. Cell counting kit-8 (CCK-8) and colony formation assay were used to assess the impact of Hsc70 on cell proliferation, while the interaction of Hsc70 with KCNA5 were evaluated by Western blot, CCK-8, and colony formation assay, respectively.
Results: Hsc70 expression was upregulated in PCOS compared to the control (p < 0.05). In granulosa cells, Hsc70 overexpression promoted cell proliferation but inhibited apoptosis (p < 0.05), whereas Hsc70 knockdown suppressed cell proliferation and promoted apoptosis (p < 0.05). Furthermore, KCNA5 expression was downregulated in PCOS (p < 0.05) and was negatively correlated with Hsc70 expression. KCNA5 knockdown promoted granulosa cell proliferation and also attenuated the Hsc70 knockdown-mediated inhibition of granulosa cell proliferation (p < 0.05).
Conclusion: Hsc70 promotes granulosa cell proliferation by interacting with KCNA5, further defining the molecular mechanism behind PCOS.

Keywords: Hsc70, KCNA5, Granulosa cells, Polycystic ovary syndrome, Apoptosis

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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