Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of glaucocalyxin B on the protein expressions of PTEN, Beclin1 and LC3 in a mouse model of transplanted cervical cancer, and its significance

Zheng Zhao¹, Zhengyang Han¹, Cong Huang², Feixiang Yang¹

¹Department of Clinical Laboratory, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, PR China; ²Department of Pharmacy, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, PR China.

For correspondence:- Feixiang Yang Email: feixiangyang1125@163.com

Accepted: 27 April 2022 Published: 31 May 2022

Citation: Zhao Z, Han Z, Huang C, Yang F. Effect of glaucocalyxin B on the protein expressions of PTEN, Beclin1 and LC3 in a mouse model of transplanted cervical cancer, and its significance. Trop J Pharm Res 2022; 21(5):951-956 doi: 10.4314/tjpr.v21i5.7

© 2022 The authors.
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Abstract

Purpose: To determine the effect of glaucocalyxin B (GLB) on the protein expressions of PTEN, Beclin1 and LC3 in a mouse model of transplanted cervical cancer, and its significance.

Methods: A mouse model of transplanted cervical cancer was established in female BALB/C mice. The model mice were divided into control group, low-dose GLB group and high-dose GLB group. Mice in low-dose and high-dose groups were given intraperitoneal injection of low-dose GLB and high-dose GLB, respectively. The volume and weight of transplanted tumor were measured and compared between the two groups. Serum levels of CEA and CA125 were assayed by enzyme-linked immunosorbent assay (ELISA). The expressions of phosphatase and tensin homolog (PTEN), autophagy-related factor microtubule-associated protein-1 (Beclin-1), microtubule-associated protein 1 light chain 3 (LC3), apoptosis-related protein p53, and Bax were determined using SABC immunohistochemical operation.

Results: On days 5, 10 and 15, the volume and weight of transplanted tumor, and levels of CA125 and CEA in low- and high-dose GLB groups were significantly and dose-dependently lower than those in control group (p < 0.05). Results from immunohistochemistry showed that the protein expression levels of PTEN, Beclin-1, LC3, p53 and Bax were significantly and dose-dependently higher in low- and high-dose GLB groups than in the control group (p < 0.05).

Conclusion: Glaucocalyxin B significantly and dose-dependently induces apoptosis of cervical cancer cells and inhibits their growth by regulating the protein expressions of PTEN, Beclin1 and LC3. Thus, glaucocalyxin B is a potential adjunct therapy in the management of cervical cancer.

Keywords: Glaucocalyxin B, Mouse, Cervical cancer transplanted tumor, PTEN, Beclin1, LC3