Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of enalapril maleate-folic acid tablets on inflammatory response and myocardial endoplasmic reticulum stress-related factors in hypertensive rats

Yugang Zu¹, Xiaoqiong Zhang², Cuina Feng¹

¹Department of Cardiovascular Medicine, Affiliated Hospital of Hebei University, China; ²Department of Cardiovascular Medicine, Baoding Second Hospital, Baoding 071000, Hebei Province, China.

For correspondence:- Cuina Feng Email: cft6qk@163.com

Accepted: 16 April 2022 Published: 31 May 2022

Citation: Zu Y, Zhang X, Feng C. Effect of enalapril maleate-folic acid tablets on inflammatory response and myocardial endoplasmic reticulum stress-related factors in hypertensive rats. Trop J Pharm Res 2022; 21(5):1003-1008 doi: 10.4314/tjpr.v21i5.14

© 2022 The authors.
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Abstract

Purpose: To determine the effect of enalapril maleate folate on inflammatory reaction and myocardial endoplasmic reticulum stress-related factors in hypertensive rats.

Methods: Eighty (80) hypertensive rats with SBP > 140 mmHg were equally assigned to control and study groups. Rats in control group were given normal saline by gavage, while the observation group was given enalapril maleate powder (10 mg/kg/day). After 4 weeks of treatment, 2 mL of inferior vena cava blood was collected from each of the two rat groups. The level of homocysteine (Hcy) was determined with Hcy assay kit, while C-reactive protein (CRP) levels in patients were determined by latex immunoturbidimetry. Serum FBG was assessed using automatic biochemical analyzer. The expression levels of GRP78, CRP94, chop and caspase-12 in aortic smooth muscle cells were assayed immunohistochemically.

Results: Central arterial pressure (MAP), aortic media thickness, lvwi, HWI FBG and CRP were significantly higher in the study rats, while Hcy level was lower, than in controls (p < 0.05). There were significantly lower levels of glucose regulatory protein 78 (GRP78), CRP94, CHOP and caspase-12 in study rats than in control rats (p < 0.05).

Conclusion: Enalapril maleate-folate slows down inflammatory reaction by reducing the levels of Hcy, CRP and FBG. It inhibits the expressions of GRP78, CRP94, CHOP and caspase-12 in myocardium, reduces damage to myocardial cells, and alleviates or reverses left ventricular hypertrophy in rats with high blood pressure. This provides new insight into the research and development of other drugs.

Keywords: Enalapril maleate-folic acid, Hypertension, Inflammatory response, Endoplasmic reticulum stress factors