Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine

YuJie Liu¹, Ke Pei¹, LiYing He², MeiBian Hu¹

¹Institute of Pharmaceutical and Food engineering, Shanxi University of Chinese Medicine, Jinzhong, China; ²School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

For correspondence:- MeiBian Hu Email: hmbcdtcm@163.com

Accepted: 2 May 2022 Published: 31 May 2022

Citation: Liu Y, Pei K, He L, Hu M. Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine. Trop J Pharm Res 2022; 21(5):1019-1026 doi: 10.4314/tjpr.v21i5.16

© 2022 The authors.
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Abstract

Purpose: To investigate the spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA).

Methods: The fingerprints of 20 PRPCA batches were established using HPLC and their similarities or differences were analyzed using hierarchical cluster analysis (HCA) and principal component analysis (PCA). The inhibitory effects of MUC5AC mucin were evaluated in LPS-treated NCI-H292 cells. The spectrum-effect relationship between common chromatographic peaks and MUC5AC inhibition was established using a partial least squares-discriminant analysis (PLS-DA).

Results: Fifteen common chromatographic peaks were identified by analyzing HPLC fingerprints, with uridine, tyrosine, uracil, and inosine found as possible markers to distinguish the PRPCA from different sources. Spectrum-effect relationship analysis showed that the chromatographic peaks 5, 6, 10 (vernine), 12 (5?hydroxymethylfurfural), 14 (tryptophan) and 15 (adenosine) were closely associated with the inhibitory effect on MUC5AC mucin.

Conclusion: The spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of PRPCA was successfully established in the present study. Our findings further reveal the material basis of PRPCA and provide an effective method for its quality control.

Keywords: Pinelliae Rhizoma Praeparatum Cum Alumine, HPLC fingerprint, spectrum-effect relationship, MUC5AC mucin