Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Electrospray ionization-mass spectrometry of different extracts of the organs of Rumex cyprius and their antihepatotoxic effect

Basma G Eid¹, Nadia M Sokkar²

¹Department of Pharmacology & Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; ²Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

For correspondence:- Nadia Sokkar Email: nadia.sokkar@pharma.cu.edu.eg Tel:+201009837452

Accepted: 19 April 2022 Published: 31 May 2022

Citation: Eid BG, Sokkar NM. Electrospray ionization-mass spectrometry of different extracts of the organs of Rumex cyprius and their antihepatotoxic effect. Trop J Pharm Res 2022; 21(5):1027-1035 doi: 10.4314/tjpr.v21i5.17

© 2022 The authors.
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Abstract

Purpose: Phytochemical and biological investigations of the valuable plant, Rumex cyprius, family Polygonaceae, wildly grown in Saudi Arabia.

Methods: Chloroform, ethyl acetate and methanol extracts were prepared from the different organs of R. cyprius. The extracts were analyzed by electrospray ionization source coupled to a mass spectrometer (ESI-MS) and tandem mass spectrometer (ESI-MS/MS) at different collision energies. The plant organs (leaf, fruit and stem) were standardized on the bases of quercetin by HPLC, and determined for their hepatoprotection in tetrachloride-induced acute liver toxicity using a mouse model.

Results: Twenty-five phenolic compounds distributed between the leaf, fruit and stem of R. cyprius were identified. They were related to classes of anthraquinones, phenolic acids, flavonoid aglycones, glycosides and polyphenols. Twenty-two compounds in total were found and identified, and for the hepatoprotective effects, the leaf exhibited the best activity.

Conclusion: R. cyprius is a source of potentially active phytoconstituents and a good natural hepatoprotective drug. This study is being documented for the first time.

Keywords: Rumex cyprius, Mass spectrometry, HPLC, Antihepatotoxicity