Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Oxobedfordia acid reduces colon cancer cell viability through apoptosis induction and inhibits colon cancer growth in mice model

Songbo Ma¹, Zhi Yu², Mingliang Zhang¹, Jianwen Ma¹

¹Department of General Surgery, The Ningxia Hui Autonomous Region People’s Hospital, Yinchuan, Ningxia 750000, China; ²Department of Anesthesiology, The Ningxia Hui Autonomous Region People’s Hospital, Yinchuan, Ningxia 750000, China.

For correspondence:- Jianwen Ma Email: yanpeng.xhu@yahoo.com Tel:+869512063131

Accepted: 31 May 2022 Published: 30 June 2022

Citation: Ma S, Yu Z, Zhang M, Ma J. Oxobedfordia acid reduces colon cancer cell viability through apoptosis induction and inhibits colon cancer growth in mice model. Trop J Pharm Res 2022; 21(6):1215-1220 doi: 10.4314/tjpr.v21i6.12

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: Colon cancer is amongst the most commonly diagnosed carcinoma globally and ranks 3rd highest of all the kinds of tumors. In the present study effect of oxobedfordia acid on colon cancer cell viability and colorectal tumor growth in vivo was investigated.

Methods: Cytotoxicity of oxobedfordia acid in SW480, HCT116, and FHC cells was evaluated by MTT assay. Colon cancer in the mice was induced by implanting subcutaneously 2 x 10⁶ HT-29 cells/mouse in the right flank. Various parameters, including cell viability, tumor growth and expression levels of cancer factors, were also assessed.

Results: Treatment with oxobedfordia acid significantly reduced viability in SW480 and HCT116 cells (p < 0.05). Furthermore, oxobedfordia acid caused increased miR-331-3p levels in cells. Moreover, oxobedfordia acid caused a significant reduction in NRP2 expression and increased apoptosis induction in SW480 and HCT116 cells. Oxobedfordia acid treatment for 48 h significantly increased p53 and p-c-Jun levels, but reduced Bcl-2 expression in cells (p < 0.05). In the mouse model of colon cancer, oxobedfordia acid significantly retarded tumor growth. Furthermore, in oxobedfordia acid-treated mice, expression of miR-331-3p was elevated while NRP2 level was lowered when compared with control group (p < 0.05).

Conclusion: Oxobedfordia acid treatment suppresses colon cancer cell viability and inhibits tumor growth in mice through enhancement of miR-331-3p and reduction in NRP2 expression. Hence, oxobedfordia acid can potentially be developed as an agent for the management of colorectal cancer.

Keywords: Oxobedfordia acid, Colorectal carcinoma, Chemotherapy, Neuropilin-2