Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of sevoflurane post-conditioning on apoptosis and the expressions of Bcl-2 and Bax in lung tissue of cardiopulmonary bypass dogs

Song Chen, Junli Luo, Xuejiao Dou, Lu You, Dong Li, Hong Zhang

Department of Anesthesiology, Af?liated Hospital of Zunyi Medical University, No.149 Dalian Road, Huichuan District, Zunyi City563003, Guizhou Province, China;

For correspondence:- Hong Zhang Email: zzhy65@163.com

Accepted: 30 June 2022 Published: 31 July 2022

Citation: Chen S, Luo J, Dou X, You L, Li D, Zhang H. Effect of sevoflurane post-conditioning on apoptosis and the expressions of Bcl-2 and Bax in lung tissue of cardiopulmonary bypass dogs. Trop J Pharm Res 2022; 21(7):1383-1390 doi: 10.4314/tjpr.v21i7.4

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effects of sevoflurane post-conditioning on the expressions of Bcl-2 and Bax in lung tissues of cardiopulmonary bypass (CPB) dogs.

Methods: Twelve healthy hybrid mongrels were divided into control (C) and sevoflurane post-conditioning (S) groups. All dogs were subjected to thoracotomy in order to establish CPB. Ischemia-reperfusion was conducted in the left lung of both groups. Thereafter, sevoflurane post-conditioning was given to group S. Femoral artery blood specimens were obtained prior to CPB (T₁), as the left pulmonary artery was opened (T₂), and 2 h after CPB (T₃), for blood gas analysis. Respiration index (RI), oxygenation index (OI), and dynamic lung compliance (Cd) were calculated.

Results: When T₁ was compared with T₂ and T₃, their OI and Cd were significantly decreased in both groups, while RI showed the opposite trend (p < 0.05). Values of OI and Cd at T₃ in group S were increased significantly, relative to group C, while RI decreased (p < 0.05). There were less lung tissue inflammation and structural disorder at T₃ in Group S than in group C. Protein expressions and positive integral of Bcl-2 and Bax, and apoptosis at T₂ and T₃ in dog lung tissues of both groups were higher than those at T₁ (p < 0.05).

Conclusion: Post-conditioning with sevoflurane is lung-protective in CPB dogs. The underlying mechanism may be based on the promotion of Bcl-2 expression and inhibition of Bax expression, thereby reducing apoptosis in dog lung tissue. Further investigations to determine its suitability for clinical applications in humans are, however, required.

Keywords: Sevoflurane, Cardiopulmonary bypass, Bcl-2, Bax, Apoptosis, Pulmonary ischemia-reperfusion injury