Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Chrysoeriol alleviated inflammation in infantile pneumonia by inhibiting PI3K/AKT/mTOR signaling pathway

Yan Wang¹, Qiuyue Hong²

¹Department of Pediatrics, Hangzhou Ninth People's Hospital, Hangzhou, Zhejiang Province 311225, China; ²Department of Pediatrics, Lishui Chinese Medicine Hospital, Lishui, Zhejiang Province 323000, China.

For correspondence:- Qiuyue Hong Email: Hongqiuyue_666@163.com Tel:+865782668311

Accepted: 24 June 2022 Published: 31 July 2022

Citation: Wang Y, Hong Q. Chrysoeriol alleviated inflammation in infantile pneumonia by inhibiting PI3K/AKT/mTOR signaling pathway. Trop J Pharm Res 2022; 21(7):1431-1436 doi: 10.4314/tjpr.v21i7.11

© 2022 The authors.
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Abstract

Purpose: To assess the therapeutic effects of chrysoeriol (CHE) on pediatric pneumonia and determine the mechanism of action.

Methods: The role of chrysoeriol was investigated in a human lung fibroblasts (HFL1) cell model of pneumonia. The effects of lipopolysaccharide (LPS) and CHE on cell viability and apoptosis were determined by CCK-8 kit and flow cytometry, respectively, while oxidative stress was determined by evaluating the levels of superoxide dismutase (SOD), glutathione, r-glutamyl cysteine +glycine (GSH)-px, myeloperoxidase (MPO) and malondialdehyde (MDA). Inflammatory response was assessed by determining IL-1β, TNF-α, IL-18 and IL-10 levels using enzyme-linked immunosorbent assay (ELISA). The mechanisms of action of CHE were evaluated by immunoblot assays.

Results: Chrysoeriol increased the viability of LPS-induced pneumonia cells (p < 0.001) but decreased cell apoptosis (p < 0.001). Furthermore, chrysoeriol reduced oxidative stress and inflammation in LPS-induced pneumonia cells, and suppressed the activation of PI3K/AKT/mTOR pathway.

Conclusion: Chrysoeriol alleviates inflammation of infantile pneumonia by inhibiting PI3K/ AKT /mTOR signaling pathway. Thus, CHE is a potential drug for the management of pneumonia.

Keywords: Chrysoeriol, Pneumonia, Cell viability, Oxidative stress, PI3K/AKT/mTOR pathway