Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of a combination of oxaliplatin and fluoropyrimidine derivative molecule S-1 on expression levels of IL-9 and CysLTR-1 in a rat model of colon cancer

Yan Li¹, Bin Huang², Xiangcai Zou³, Xiongbo Guo³ , Yikun Lin⁴

¹Department of Pathology, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou 511402, China; ²Department of General Surgery, Daping Hospital, Army Medical Center of People's Liberation Army, Chongqing 400042, China; ³Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China; ⁴Department of General Surgery, Marine Corps Hospital of People's Liberation Army, Guangzhou, PR China.

For correspondence:- Xiongbo Guo Email: ep62c2@163.com

Accepted: 1 July 2022 Published: 31 July 2022

Citation: Li Y, Huang B, Zou X, Guo X, Lin Y. Effect of a combination of oxaliplatin and fluoropyrimidine derivative molecule S-1 on expression levels of IL-9 and CysLTR-1 in a rat model of colon cancer. Trop J Pharm Res 2022; 21(7):1437-1443 doi: 10.4314/tjpr.v21i7.12

© 2022 The authors.
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Abstract

Purpose: To investigate the effect of a combination of oxaliplatin and fluoropyrimidine derivative molecule S-1 on the expression levels of IL-9 and CysLTR-1 in rats with colon cancer.

Methods: One hundred and eighty healthy Spraque Dawley rats were randomly assigned to groups A (healthy control), B (model), and C (treatment) with 60 rats in each group. Western blotting was used to determine contents of CysLTR-1 and related pathway proteins, while the levels of IL-9 and IFN-γ were evaluated by enzyme-linked immunosorbent assay (ELISA). Cell Counting Kit-8 (CCK-8) was used to determine cell proliferation, while cell apoptosis was assessed using flow cytometry.

Results: Tumor volume was larger and tumor diameter was longer in group B than in group C (p < 0.05). The expression levels of CysLTR-1, LTD4, β-catenin, COX-2, IL-9 and IFN-γ were significantly up-regulated in groups B and C, relative to group A (p < 0.05). Cell proliferation was also significantly faster in groups B and C than in group A, while apoptotic rate was lower (p < 0.05).

Conclusion: Combined use of OX and S-1 inhibits CysLTR-1 expression level in colon cancer rats. Furthermore, it down-regulates IL-9 expression, thereby inhibiting the proliferation and apoptosis of tumor cells. Therefore, the combination treatment has potentials for development as a therapeutic strategy for the management of colon cancer.

Keywords: Oxaliplatin, Fluoropyrimidine derivative molecule, Colon cancer, CysLTR-1