Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

3-O-Caffeoylquinic acid in Periploca forrestii Schltr extract ameliorates collagen-induced arthritis by inducing IL17/IL23 cells in rats

Qiaoyi Ning^1,2, Xueming Yao², Ying Huang², Lei Hou², Daomin Lu², Yutao Yang³, Yamei Zhan⁴, Yiting He⁵, Wukai Ma²

¹The 2nd Clinical School, Guangzhou University of Chinese Medicine, Guangzhou 510120, China; ²Rheumatology and Immunology Department, The 2nd Hospital Affiliated with Guizhou university of Traditional Chinese Medicine, Guiyang 550003, China; ³Physical Examination Center, The 2nd Hospital Affiliated With Guizhou University of Traditional Chinese Medicine, Guiyang 550003, China; ⁴Department of Pharmacy, Guizhou Provincial People’s Hospital, Guiyang 550002, China; ⁵Intellectual Property Management and Transformation Center, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China.

For correspondence:- Wukai Ma Email: w7n3kx@163.com Tel:+8685185556970

Accepted: 19 June 2022 Published: 31 July 2022

Citation: Ning Q, Yao X, Huang Y, Hou L, Lu D, Yang Y, et al. 3-O-Caffeoylquinic acid in Periploca forrestii Schltr extract ameliorates collagen-induced arthritis by inducing IL17/IL23 cells in rats. Trop J Pharm Res 2022; 21(7):1445-1452 doi: 10.4314/tjpr.v21i7.13

© 2022 The authors.
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Abstract

Purpose: To study the therapeutic effect of 3-O-caffeoylquinic acid (3-O-CQA) from Periploca forrestii extract (PFE) on collagen-mediated arthritis (CIA) in rats, as well as the potential underlying mechanism of action.

Methods: PFE and 3-O-CQA were successively and intragastrically administered to CIA rats. Paw swelling, arthritic scores and H & E staining were used to evaluate the therapeutic effect of 3-O-CQA. Moreover, to determine the effects of PFE and 3-O-CQA on fibroblast-resembling synoviocytes obtained from arthritic subjects (RAFLS), the viability of RAFLS cultured in vitro was measured with MMT, while apoptotic lesions were analyzed by flow cytometry. The levels of IL-6 in CIA and RAFLS were determined by enzyme-linked immunosorbent assay (ELISA), while quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunoblotting were used to assess their mRNA and polypeptide levels, respectively.

Results: PFE in 3-O-CQA ameliorated swelling and reduced arthritic scores in CIA rat model, and also decreased cytokine levels (p < 0.05). By decreasing mRNA and protein expressions, 3-O-CQA repressed the phosphorylation of STAT3 and JAK2 as well as the protein levels of IL-23 and RORγt (p < 0.05).

Conclusion: The results of this study show that CIA and RAFLS are ameliorated in rats by 3-O-CQA in PFE through regulation of IL17/ IL23 and Th17 cells. Thus, 3-O-CQA affords a therapeutic strategy for the management of collagen-induced arthritis.

Keywords: Arthritis, Periploca forrestii Schltr extract, 3-O-Caffeoylquinic acid, Interleukin (IL)-17, IL-23, Th17 cells