Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Patchouli alcohol suppresses inflammation and autophagy, and reduces osteoclast formation in periodontitis via regulation of ERK and NF-κB signaling pathways

Fei Ye, Changqing Zhang

Department of Stomatology, Wuhan Hankou Hospital, Wuhan City, Hubei Province 430012, China;

For correspondence:- Changqing Zhang Email: changq_zh@163.com Tel:+862782283158

Accepted: 18 July 2022 Published: 28 August 2022

Citation: Ye F, Zhang C. Patchouli alcohol suppresses inflammation and autophagy, and reduces osteoclast formation in periodontitis via regulation of ERK and NF-κB signaling pathways. Trop J Pharm Res 2022; 21(8):1607-1613 doi: 10.4314/tjpr.v21i8.5

© 2022 The authors.
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Abstract

Purpose: To evaluate the effect of patchouli alcohol (PA) on periodontitis and reveal its possible mechanism.

Methods: Primary human periodontal membrane cells (PDLC) were isolated and treated with lipopolysaccharide (LPS) to establish a periodontitis model. The MTT, flow cytometry and immunofluorescence assay were used to determine the effect of PA on cell viability and apoptosis in PDLC exposed to LPS. The inflammation status and osteoclast formation were measured using enzyme-linked immunosorbent assay (ELISA) and TRAP staining. Immunoblot assays were performed to analyze the effect of LPS on ERK and NF-κB pathways.

Results: Patchouli alcohol (PA) improved LPS-induced PDLC growth arrest (p < 0.001). In addition, PA relieved LPS - induced autophagy of PDLCs (p < 0.001). Further data confirmed that PA inhibited lPS-induced inflammation of PDLCs. In addition, PA reduced osteoclast formation, and suppressed inflammation and autophagy, and also reduced osteoclast formation by regulating ERK and NF-κB pathways.

Conclusion: Patchouli alcohol inhibits inflammation and autophagy, and decreases osteoclast formation in periodontitis via the regulation of ERK and NF-κB signaling pathways. Thus, this compound has potentials for development for the treatment of periodontitis; however, in vivo studies should first be undertaken.

Keywords: Periodontitis, Patchouli alcohol (PA), Lipopolysaccharide (LPS), Osteoclast formation, ERK and NF-κB pathways