Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effects of suppressor of cytokine signaling 3 (SOCS3) on the development of colon cancer via regulation of HIF-1α

Fang Chen¹, Yifan Long², Cuiyun Guo², Hui Zou³, Yingying Wang³, Kunbo Dong³

¹Department of Spleen and Stomach, Yantai Hospital of traditional Chinese Medicine, Yantai, China; ²Department of Gastroenterology, The 970th Hospital of The PLA Joint Logistics Support Force l, Yantai, China; ³Department of Endoscopy, Yantai Hospital of traditional Chinese Medicine, Yantai, China.

For correspondence:- Kunbo Dong Email: dkbdym1@163.com Tel:+8618253541913

Accepted: 25 August 2022 Published: 30 September 2022

Citation: Chen F, Long Y, Guo C, Zou H, Wang Y, Dong K. Effects of suppressor of cytokine signaling 3 (SOCS3) on the development of colon cancer via regulation of HIF-1α. Trop J Pharm Res 2022; 21(9):1873-1878 doi: 10.4314/tjpr.v21i9.9

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the influence of suppressor of cytokine signaling 3 (SOCS3) on rats with colon cancer (CC).

Methods: Sprague-Dawley (SD) rats were randomly divided into CC group and control group. CC models were constructed. The expression of SOCS3 in CC tissues was determined by quantitative real time-polymerase chain reaction (qRT-PCR). Hematoxylin-eosin staining (H&E) was used to examine colon tissue morphology, while immunohistochemistry (IHC) staining assay was performed to determine the expression of SOCS3 protein in colon tissues. The content of HIF-1α, phosphorylated phosphatidylinositol 3-hydroxy kinase (p-PI3K), and phosphorylated protein kinase B (p-AKT) proteins was determined by Western blotting (WB).

Results: Compared with that in the control group, the number of tumors in the CC group was significantly increased (p < 0.05). Protein and messenger ribonucleic acid (mRNA) expressions of SOCS3 were down-regulated in CC group (p < 0.05), while protein expressions of p-PI3K, p-AKT and HIF-1α were significantly elevated in CC group (p < 0.05).

Conclusion: SOCS3 is poorly expressed in CC rats, and promotes the expression of HIF-1α by activating PI3K/AKT signaling pathway. The findings, thus, provide a probable strategy for management of colon cancer.

Keywords: Colon cancer, Suppressor of cytokine signaling protein 3 (SOC3), Hypoxia inducible factor-1α