Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Total phenolic and flavonoid contents of Mentha spicata leaves aqueous extracts in different regions of Algeria and their antioxidant, and antidiabetic activities

Abdelbasset Kaddour^1,3, Djilani Ghemam Amara^1,2 , Younes Moussaoui^3,4, Ahmed Elkhalifa Chemsa^2,5, Zaid Alia^1,2, Abasse Kamarchou⁶

¹Laboratory of Biology, Environment and Health,University of El Oued, Algeria; ²Department of Biology, Faculty of Life and Natural Sciences, University of El Oued, Algeria; ³Laboratory of Organic Chemistry (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, Algeria; ⁴Faculty of Sciences of Gafsa, University of Gafsa, Tunis; ⁵El Oued University, Laboratory of Biodiversity and Application of Biotechnology in Agriculture; ⁶Department of Chemistry, University Ouargla, Algeria.

For correspondence:- Djilani Amara Email: ghemamamaradjilani@gmail.com Tel:+213658943163

Accepted: 31 August 2022 Published: 30 September 2022

Citation: Kaddour A, Amara DG, Moussaoui Y, Chemsa AE, Alia Z, Kamarchou A. Total phenolic and flavonoid contents of Mentha spicata leaves aqueous extracts in different regions of Algeria and their antioxidant, and antidiabetic activities. Trop J Pharm Res 2022; 21(9):1907-1913 doi: 10.4314/tjpr.v21i9.14

© 2022 The authors.
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Abstract

Purpose: To evaluate the total phenolic and flavonoid contents of Mentha spicata (MS) leaves aqueous extracts obtained from different regions (El-Oued, Tebessa and El-Tarf) of Algeria, as well as their in vitro antioxidant and antidiabetic activities.

Methods: Aqueous extracts were obtained from the air-dried leaves of MS by maceration followed by filtration and evaporation using a rotary evaporator. Folin-Ciocalteu and Aluminium chloride colorimetric techniques were used to determine the total phenolic and flavonoid contents, respectively. The radical DPPH, ABTS, and FRAP tests were used to determine their antioxidant activities, while the in vitro inhibition percentages of α-amylase and α-glucosidase enzymes were used to assess the anti-diabetic activity.

Results: The El-Oued's extract exhibited the highest total phenolic content (108.94 mg GAE/g dry extract (DE)), while the highest total flavonoid content (0.039 mg QE/g DE) was found in El-Tarf's extract. The radical DPPH and FRAP scavenging activity of the El-Oued extract exhibited the highest inhibition activities (IC₅₀ = 102.5 and 289.5 µg/mL), respectively, while the ABTS inhibition activity of the El-Tarf extract exhibited a maximum IC₅₀ value of 111 ± 2.8 µg/mL. The MS extract of the El-Oued region had the highest α-amylase and α-glucosidase enzyme activities with IC₅₀ values of 121.4 and 216.9 µg/mL, respectively.

Conclusion: The leaves of Mentha spicata exhibit high phenolic and flavonoid contents, along with significant antioxidant and antidiabetic properties. This study reveals that Mentha spicata flavonoid and phenolic contents as well as other properties vary by region.

Keywords: Mentha spicata, Antidiabetic, Antioxidant, ?-Amylase, ?-Glucosidase