Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Comparative study of the analgesic effects of Bungarus fasciatus snake venom from Vinh Phuc and Tien Giang Provinces of Vietnam

Thien Vu Tran¹, Trang Thuy Thi Nguyen², Anh Ngoc Hoang³, Yuri N Utkin⁴

Faculty of Pharmacy, Nguyen Tat Thanh University, Vietnam; ¹Tra Vinh University, Tra Vinh City, Vietnam; ³Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam; ⁴Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

For correspondence:- Yuri Utkin Email: utkin@ibch.ru

Accepted: 30 August 2022 Published: 30 September 2022

Citation: Tran TV, Nguyen TT, Hoang AN, Utkin YN. Comparative study of the analgesic effects of Bungarus fasciatus snake venom from Vinh Phuc and Tien Giang Provinces of Vietnam. Trop J Pharm Res 2022; 21(9):1915-1921 doi: 10.4314/tjpr.v21i9.15

© 2022 The authors.
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Abstract

Purpose: To determine the analgesic activity of Bungarus fasciatus venoms and their fractions from two Vietnamese Provinces.

Methods: Male Swiss Albino mice were randomly divided into three groups containing 8 to 10 mice each. Control group was injected subcutaneously with normal saline, standard group received aspirin solution (50 mg/kg) perorally, and study group received a solution of crude venom or isolated fractions in physiological saline. To determine analgesic activity, acetic acid writhing and tail immersion tests were used. The venoms were separated by liquid chromatography and the analgesic activity of the fractions was analyzed.

Results: Both venoms showed analgesic effect in the acetic acid writhing test, but only the venom from Tien Giang showed analgesic effect in the tail immersion test. The bioactive fractions of Vinh Phuc and Tien Giang venoms were significantly different, with most of Vinh Phuc venom fractions being more active (p < 0.05). Thus, 35 min after the injection, the number of writhings decreased from 15 - 16 in the control to 0.85 ± 0.34 for the BF-4VS (Vinh Phuc) fraction compared to 2.67 ± 1.20 (p < 0.05) for the BF-4DT (Thien Giang) fraction. Two proteins with analgesic activity were isolated from Vinh Phuc venom, and one with greater activity matched the known B. fasciatus phospholipase A2.

Conclusion: The analgesic activity of two samples of B. fasciatus venoms from two different provinces in Vietnam reveal that their pharmacological profiles differ. The isolates can be explored as leads in the development of new analgesic agents.

Keywords: Analgesic activity, Krait Bungarus fasciatus, Phospholipase A2, Venom