Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Investigation of the cardiac depressant effect of Caralluma tuberculata N.E.Br on isolated rabbit heart

Mater H Mahnashi¹, Taseer Ahmad² , Alamgeer³, Muhammad Naveed Mushtaq², Muhammad Nasir Hayat Malik², Maira Ahmad², Adil Javed⁴, Mohammed M Jalal⁵, Malik A Altayar⁵, Abdullah Albloshi⁶

¹Department of Pharmaceutical Chemistry, School of Pharmacy, Najran University, Najran, Saudi Arabia; ²Laboratory of Cardiovascular research and Integrative Pharmacology, College of Pharmacy, University of Sargodha, Lahore; ³Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore; ⁴Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus-22060, KPK, Pakistan; ⁵Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk; ⁶Department of Anatomy and Histology, Faculty of Medicine, Albaha University, Saudi Arabia.

For correspondence:- Taseer Ahmad Email: taseer.ahmad@uos.edu.pk

Accepted: 26 September 2022 Published: 28 October 2022

Citation: Mahnashi MH, Ahmad T, Alamgeer , Mushtaq MN, Malik MN, Ahmad M, et al. Investigation of the cardiac depressant effect of Caralluma tuberculata N.E.Br on isolated rabbit heart. Trop J Pharm Res 2022; 21(10):2125-2130 doi: 10.4314/tjpr.v21i10.12

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the histopathological and cardiac depressant effect of the aqueous methanol extract of Caralluma tuberculata N.E. Br (AMECT) (family: Asclepiadaceae)’ and to determine if there is a scientific basis for its cardiovascular diseases-related folkloric use.

Methods: The effect of AMECT in different concentrations ranging from 0.00001 to 1.0 mg/mL were evaluated in isolated perfused rabbit heart to assess their effect on the force of contraction and heart rate using Langendorff’s apparatus. Atropine and adrenaline were used to identify the underlying mechanism of response produced by AMECT. The extract was studied for its possible mechanism in the absence and presence of atropine and adrenaline. In addition, sub-chronic toxicity and histopathological study of heart tissues in rats were assessed by administering 500 mg/kg of extract.

Results: At all concentrations, AMECT produced significant (p < 0.001) negative ionotropic and negative chronotropic effects. The most significant effect was observed at 0.001 mg/mL and higher concentrations hence 0.001 mg/mL was selected for further studies. Pre-incubation with atropine did not significantly inhibit the effects of AMECT. However, AMECT significantly (p < 0.01) blocked the cardiac stimulant effect of adrenaline. In the histopathological studies, AMECT did not produce any significant cellular changes or signs of toxicity in the sub-chronic toxicity study.

Conclusion: The cardiac-depressant responses of AMECT may involve the β-adrenergic receptors in the myocardium of isolated rabbit heart thus confirming the rationale for its use in ethnomedicine for cardiac diseases.

Keywords: Caralluma tuberculata N.E. Br, Cardiac depressant effect, Atropine, Adrenaline, Isolated rabbit heart, Histopathology