Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Regulatory effect of resveratrol on the expressions of factors and surface markers in alveolar macrophages of Aspergillus fumigatus-infected COPD rat model, and the associated mechanism

Limei Du^1,2 , Xinglan Huo², Ying Liu³

¹Department of Emergency, TianJin Medical University General Hospital, Tianjin 300052, China; ²Department of Infection, Tianjin Beichen Hospital, Tianjin 300400, China; ³Department of Respiratory, Tianjin Beichen Hospital, Tianjin 300400, China.

For correspondence:- Limei Du Email: orangetea2022@163.com

Accepted: 27 September 2022 Published: 28 October 2022

Citation: Du L, Huo X, Liu Y. Regulatory effect of resveratrol on the expressions of factors and surface markers in alveolar macrophages of Aspergillus fumigatus-infected COPD rat model, and the associated mechanism. Trop J Pharm Res 2022; 21(10):2139-2146 doi: 10.4314/tjpr.v21i10.14

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the influence of resveratrol on chronic obstruction in Aspergillus fumigatus infection-induced chronic lung disease (COPD) in rats, and the process involved.

Methods: Seventy-five Sprague Dawley SD) rats were assigned to blank control, COPD model, Aspergillus fumigatus-infected COPD model, low (100 mg/kg) and high-dose (200 mg/kg) resveratrol groups, with 15 rats in each group. Enzyme-linked immunosorbent assay (ELISA) was used to assay IL-6, IL-8 and TNF-α in bronchoalveolar lavage fluid (BALF), while lung protein concentrations of AMPK and PGC-1α were assayed by immunoblotting.

Results: In low- and high-dose resveratrol groups, inflammatory factor levels were significantly lower than the corresponding levels in COPD model and Aspergillus fumigatus-infected COPD model (p < 0.05), while lung tissue proteins of AMPK, PGC-1α, and AQP5 of the rats given high-dose resveratrol were significantly raised, relative to corresponding levels in rats given low-dose resveratrol (p < 0.05).

Conclusion: Resveratrol modulates expression levels of secretory factors and surface markers in alveolar macrophages in COPD model rats infected with Aspergillus fumigatus. Resveratrol exerts this effect through the regulation of AMPK/PGC-1α signaling pathway.

Keywords: COPD, Resveratrol, Adenylate-activated protein kinase, Peroxisome proliferator-activated gamma receptor coactivator 1-Î±, Alveolar macrophages