Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Characteristics of basal gastric juice in Helicobacter pylori-associated gastritis before and after eradication therapy

Sam Phan Hai Nguyen¹, Tho Chau Minh Vinh Do², Tam Thai Thanh Tran¹, Tho Kieu Anh Pham¹, Van De Tran³ , Kien Trung Nguyen¹

¹Department of Physiology, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, Can Tho 900000, Vietnam; ²Department of Drug Quality Control-Analytical Chemistry-Toxicology, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, Can Tho 900000, Vietnam; ³Department of Drug Administration, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, Can Tho 900000, Vietnam.

For correspondence:- Van De Tran Email: tvde@ctump.edu.vn Tel:+84943848691

Accepted: 26 September 2022 Published: 28 October 2022

Citation: Nguyen SP, Do TC, Tran TT, Pham TK, De Tran V, Nguyen KT. Characteristics of basal gastric juice in Helicobacter pylori-associated gastritis before and after eradication therapy. Trop J Pharm Res 2022; 21(10):2241-2246 doi: 10.4314/tjpr.v21i10.27

© 2022 The authors.
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Abstract

Purpose: To evaluate the characteristics of basal gastric juice in Helicobacter pylori-positive patients before and after Helicobacter pylori eradication therapy.

Methods: This was a cross-sectional descriptive study on 150 gastritis patients admitted at the Hospital of Can Tho University of Medicine and Pharmacy. The patients were divided into 2 groups: study group (Helicobacter pylori gastritis patients) and control group (non-Helicobacter pylori gastritis patients). The pH, HCO₃^- concentration, and activities and concentrations of pepsin, lipase, and amylase were determined before and after treatment in study group.

Results: Patients with abnormal gastric juice comprised 76 % of the study population. Mean gastric potential of hydrogen (pH) was 2.31 (range: 1.64 - 7.68), while median concentration of HCO₃^- was 4.06 mmol/L (range: 0 - 73.04 mmol/L). The concentrations of pepsin, lipase, and amylase were 8.93, 0.93 and 1.38 ppm, respectively. Activities of pepsin, lipase, and amylase were 2.23, 0.28 and 0.04 U/mL, respectively. After the successful eradication of Helicobacter pylori, pH and HCO₃^- levels decreased, and there were significant differences in activities of pepsin and lipase before and after treatment (p < 0.05). Moreover, the levels of these parameters differed between patients in whom successful eradication was achieved and those in whom eradication failed (p < 0.05). The concentrations and activities of pepsin and lipase were statistically different between pre-treatment and post-treatment stages in both successful and failed Helicobacter pylori eradication categories (p < 0.05).

Conclusion: Basal gastric juice differs significantly between Helicobacter pylori-positive and Helicobacter pylori-negative patients. Intragastric ammonia produced by H. pylori may have a role in the increased pH of gastric juice.

Keywords: Gastric juice, Helicobacter pylori, Gastritis, Intragastric ammonia, Enzyme activities