Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Chikusetsu saponin IVA induces apoptosis and mitochondrial dysfunction of benign prostatic hyperplasia epithelial cell line (BPH-1) by inhibiting JAK/STAT3 signaling pathway

Jian Gao, Yuan Zhang

Department of Urology, Traditional Chinese Medicine Hospital of Qinhuangdao, Qinhuangdao City, Hebei Province 066000, China;

For correspondence:- Yuan Zhang Email: y_zhang0620@163.com Tel:+863358355293

Accepted: 10 October 2022 Published: 30 November 2022

Citation: Gao J, Zhang Y. Chikusetsu saponin IVA induces apoptosis and mitochondrial dysfunction of benign prostatic hyperplasia epithelial cell line (BPH-1) by inhibiting JAK/STAT3 signaling pathway. Trop J Pharm Res 2022; 21(11):2317-2322 doi: 10.4314/tjpr.v21i11.7

© 2022 The authors.
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Abstract

Purpose: To determine the potential effect of Chikusetsu saponin IVA (CS-IVA) on benign prostatic hyperplasia (BPH), as well as the mechanism of action.

Methods: Benign prostatic hyperplasia epithelial cell response to treatment by CS-IVA was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, and cell cycle analysis. Flow cytometry and immunoblot assay were used to assess the effect of CS-IVA on cell apoptosis. Mitochondrial damage was examined by JC-1 staining and immunoblot, while immunoblot assay was also performed to determine the effect of CS-IVA on JAK/STAT3 pathway.

Results: CS-IVA inhibited the growth and cycle progression of BPH-1 cell but promoted cell apoptosis (p < 0.01). It also exacerbated mitochondrial damage in BPH-1 cell lines (p < 0.01). With regard to the mechanism of action, CS-IVA inhibited JAK/STAT3 pathway in BPH-1 cells (p < 0.01).

Conclusion: CS-IVA induces apoptosis and mitochondrial dysfunction of BPH-1 cells by inhibiting JAK/STAT3 pathway, and thus it is a potential drug for the treatment of BPH. However, in vivo studies on the effect of CS-IVA are are required to validate these results

Keywords: Benign prostatic hyperplasia (BPH), Chikusetsu saponin IVA (CS-IVA), BPH-1, Mitochondrial dysfunction, JAK/STAT3 pathway